##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294943_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4161823 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.901861996533732 31.0 31.0 34.0 28.0 34.0 2 30.975136616814314 31.0 31.0 34.0 28.0 34.0 3 31.136583896047476 33.0 31.0 34.0 28.0 34.0 4 34.57900420080335 37.0 35.0 37.0 32.0 37.0 5 34.82903141243633 37.0 35.0 37.0 32.0 37.0 6 34.824667459428234 37.0 35.0 37.0 32.0 37.0 7 34.82888580316847 37.0 35.0 37.0 32.0 37.0 8 34.8738701766029 37.0 35.0 37.0 32.0 37.0 9 36.56426402564453 39.0 37.0 39.0 32.0 39.0 10 36.60299488949914 39.0 37.0 39.0 32.0 39.0 11 36.60681460984765 39.0 37.0 39.0 32.0 39.0 12 36.54291448723312 39.0 37.0 39.0 32.0 39.0 13 36.427232489224075 39.0 37.0 39.0 32.0 39.0 14 37.67107563200069 40.0 38.0 41.0 32.0 41.0 15 37.73561754067869 40.0 38.0 41.0 32.0 41.0 16 37.69546566492616 40.0 38.0 41.0 32.0 41.0 17 37.66161895880723 40.0 38.0 41.0 32.0 41.0 18 37.6289806173881 40.0 38.0 41.0 32.0 41.0 19 37.74858926004301 40.0 38.0 41.0 32.0 41.0 20 37.77179783955253 40.0 38.0 41.0 32.0 41.0 21 37.71382252440817 40.0 38.0 41.0 32.0 41.0 22 37.69031023183831 40.0 38.0 41.0 32.0 41.0 23 37.643168630669784 40.0 38.0 41.0 31.0 41.0 24 37.69836487520012 40.0 38.0 41.0 31.0 41.0 25 36.615047300185516 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14651225389437883 1101 2 -0.07546625353617742 1101 3 -0.21964342677199866 1101 4 -0.3090182029034807 1101 5 -0.30013207217222515 1101 6 -0.3644475992169518 1101 7 -0.34686454382819676 1101 8 -0.3479371880575073 1101 9 -0.15794358504830797 1101 10 0.008971606663223497 1101 11 0.019689003165645147 1101 12 0.07009090528541861 1101 13 0.01647758120642351 1101 14 -0.008098628133730301 1101 15 0.0370782310484401 1101 16 0.06312854149945935 1101 17 -0.11920343704923653 1101 18 -0.054194911091720144 1101 19 -0.1240979255809691 1101 20 -0.14735721241269317 1101 21 -0.07568636779158311 1101 22 -0.10939900783093748 1101 23 -0.08537964602655279 1101 24 -0.08676266174914815 1101 25 -0.08741556891904878 1102 1 -0.534348042964595 1102 2 -0.48174519256228976 1102 3 -0.7287115334644234 1102 4 -0.8124091367241277 1102 5 -0.7099554268773716 1102 6 -0.8004652363292664 1102 7 -0.6771236795141462 1102 8 -0.5769708305533641 1102 9 -0.43032939185824404 1102 10 -0.3903312100030476 1102 11 -0.3579167612806913 1102 12 -0.3814422709828236 1102 13 -0.35124160617038314 1102 14 -0.38924834678199716 1102 15 -0.3866044486025615 1102 16 -0.3956737950948934 1102 17 -0.5301882753544476 1102 18 -0.4710720791858449 1102 19 -0.5540148352696264 1102 20 -0.5177871434924484 1102 21 -0.5186062637800717 1102 22 -0.6386866527585013 1102 23 -0.5499541654863691 1102 24 -0.5056638684575105 1102 25 -0.5838948243012965 1103 1 -0.5157717647776572 1103 2 -0.4576407775328306 1103 3 -0.5928423202183772 1103 4 -0.6990102141773136 1103 5 -0.6080748197692429 1103 6 -0.5528166759438449 1103 7 -0.5375336793705259 1103 8 -0.4086954267593583 1103 9 -0.33077303523099033 1103 10 -0.30485607501974243 1103 11 -0.2976558171404946 1103 12 -0.18396899441751913 1103 13 -0.26471096685975226 1103 14 -0.3751138680720345 1103 15 -0.2719926264095136 1103 16 -0.3635538846351025 1103 17 -0.3547928690019617 1103 18 -0.4644857951841459 1103 19 -0.40703067213247124 1103 20 -0.3469993142509864 1103 21 -0.3466753430265328 1103 22 -0.35573071314341576 1103 23 -0.2845607511350181 1103 24 -0.30750735312152955 1103 25 -0.3265717107720221 1104 1 -0.41992365620881955 1104 2 -0.37068783175232056 1104 3 -0.5404155553681811 1104 4 -0.5310752218237909 1104 5 -0.4900676746817467 1104 6 -0.42935536909395466 1104 7 -0.3882692136716557 1104 8 -0.41378896280617283 1104 9 -0.34763365383863487 1104 10 -0.23403519140633477 1104 11 -0.3412823580474793 1104 12 -0.2795011744685567 1104 13 -0.36588650316108584 1104 14 -0.4199112075350584 1104 15 -0.38773411495022714 1104 16 -0.4193606878429037 1104 17 -0.3938123139121714 1104 18 -0.3872281209367401 1104 19 -0.39677614694848984 1104 20 -0.3018718304065544 1104 21 -0.49594903195668394 1104 22 -0.39834682409988886 1104 23 -0.480763002880515 1104 24 -0.40783590248829427 1104 25 -0.4116172870672301 1105 1 -0.5289372071522358 1105 2 -0.5021680532750068 1105 3 -0.5644189447919956 1105 4 -0.5382237327054185 1105 5 -0.5268796167135648 1105 6 -0.472217167753044 1105 7 -0.4937634717508814 1105 8 -0.4793399311499158 1105 9 -0.4292568066260287 1105 10 -0.5500036831646753 1105 11 -0.5027556043998018 1105 12 -0.46718698641110734 1105 13 -0.7836147062730632 1105 14 -0.6806562679146921 1105 15 -0.6971444663523485 1105 16 -0.6546145907961076 1105 17 -0.47824202491782586 1105 18 -0.30523387662088197 1105 19 -0.6313749396155757 1105 20 -0.4692221641811827 1105 21 -0.4333539413755503 1105 22 -0.4547589688946587 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19 0.13361687938089517 1108 20 0.21150890991052052 1108 21 0.2483836463107849 1108 22 0.17374470747940052 1108 23 0.25189980140839197 1108 24 0.17205836409321051 1108 25 0.24760125592935367 1109 1 0.2490023326658637 1109 2 0.1712775392099175 1109 3 0.23955188022653218 1109 4 0.2927600147543714 1109 5 0.27328240763200284 1109 6 0.29861117508912827 1109 7 0.18533509859987163 1109 8 0.2821998349144934 1109 9 0.23479546898976622 1109 10 0.07613137855682339 1109 11 0.015079359133878256 1109 12 0.09878110784919869 1109 13 0.17439781435938784 1109 14 0.16552091146451886 1109 15 0.1725350598023212 1109 16 0.16001635696200367 1109 17 0.24341706005655084 1109 18 0.22441990293543057 1109 19 0.23593251735859866 1109 20 0.1808717357443328 1109 21 0.1785724090923111 1109 22 0.19960017495385785 1109 23 0.2389747799857247 1109 24 0.18256727879020218 1109 25 0.10526164247595915 1110 1 0.21458518704442042 1110 2 0.18674955494928014 1110 3 0.22344171076459673 1110 4 0.2290019442697897 1110 5 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2210 2 0.4556837584280018 2210 3 0.5614113427903753 2210 4 0.6843689032913289 2210 5 0.6080534988394177 2210 6 0.5905354342582498 2210 7 0.5416620910721619 2210 8 0.530947260582245 2210 9 0.47250496803967224 2210 10 0.5112549749470361 2210 11 0.526440756812832 2210 12 0.6048395915808698 2210 13 0.5841140058198491 2210 14 0.7234673981196309 2210 15 0.5962432632386765 2210 16 0.6392729105161905 2210 17 0.5240040180474068 2210 18 0.5812669156405903 2210 19 0.5868147246071658 2210 20 0.5446701224392427 2210 21 0.5661803456013317 2210 22 0.558356681748549 2210 23 0.6341615324168188 2210 24 0.604169620997375 2210 25 0.6637626672029739 2211 1 0.39789110672811745 2211 2 0.32425916882395356 2211 3 0.4405855144805493 2211 4 0.5042700760182512 2211 5 0.4665039346151616 2211 6 0.4880673663634312 2211 7 0.3867036279450531 2211 8 0.35873274067044747 2211 9 0.3839304541766069 2211 10 0.4283848177866503 2211 11 0.38804088916035084 2211 12 0.34222309819612917 2211 13 0.4114183389512931 2211 14 0.49103441538568404 2211 15 0.4152253177916734 2211 16 0.40603020496338615 2211 17 0.5256272011495753 2211 18 0.5562541755873198 2211 19 0.4694302297421089 2211 20 0.40015901890242134 2211 21 0.46369271907354204 2211 22 0.4318754964999769 2211 23 0.3917341340967795 2211 24 0.3683886038544486 2211 25 0.32379900315334 2212 1 0.29355008980897423 2212 2 0.2536315942138607 2212 3 0.3695753710383016 2212 4 0.4247310032826519 2212 5 0.4374860320699625 2212 6 0.42473598415379143 2212 7 0.4253511889113284 2212 8 0.3539188288096824 2212 9 0.30698730009975606 2212 10 0.32040520659889893 2212 11 0.2681369993300251 2212 12 0.3140630196184375 2212 13 0.3606673103530369 2212 14 0.37157683320892687 2212 15 0.290913664219552 2212 16 0.2735241716331913 2212 17 0.2983332049524279 2212 18 0.3541149521526705 2212 19 0.31808085805422337 2212 20 0.33650117342588715 2212 21 0.3975878827103898 2212 22 0.41006497330663194 2212 23 0.31426113691029656 2212 24 0.3969168228246147 2212 25 0.2655599767744832 2213 1 0.44379375495218554 2213 2 0.45267382853437965 2213 3 0.5547302942976202 2213 4 0.5913568891722107 2213 5 0.5573349101616571 2213 6 0.5343284968031128 2213 7 0.4705392028583546 2213 8 0.5280483776869005 2213 9 0.49508135222341565 2213 10 0.461891199858421 2213 11 0.4724676265675072 2213 12 0.37363062686459614 2213 13 0.3992546642340571 2213 14 0.4687253830153679 2213 15 0.4433157976367781 2213 16 0.5129189849009279 2213 17 0.5398864864941757 2213 18 0.5160525051312703 2213 19 0.4226325753618738 2213 20 0.4974206634331395 2213 21 0.5260516999659046 2213 22 0.5091243938117884 2213 23 0.5195996319248479 2213 24 0.5039503417104854 2213 25 0.5264142175383597 2214 1 0.3954821762498213 2214 2 0.4969607404306977 2214 3 0.5652144506988925 2214 4 0.60798049801042 2214 5 0.524562169672059 2214 6 0.5725294245246673 2214 7 0.5072720706144338 2214 8 0.47202728492950996 2214 9 0.3793769340456521 2214 10 0.4744332406741876 2214 11 0.4342639095706389 2214 12 0.40649335366700967 2214 13 0.41808240133704544 2214 14 0.43573100241814444 2214 15 0.44838206566879535 2214 16 0.4667359665583035 2214 17 0.5135649965096505 2214 18 0.5374378789611001 2214 19 0.5508509699545598 2214 20 0.47580498128625237 2214 21 0.45391151390665385 2214 22 0.4819364690909822 2214 23 0.44827481540767167 2214 24 0.44278754175181945 2214 25 0.3604785555635175 2215 1 0.4973284231721955 2215 2 0.5178524575361152 2215 3 0.5960054497955269 2215 4 0.6738405518349637 2215 5 0.5770200836558885 2215 6 0.5852757314580046 2215 7 0.5719451725950009 2215 8 0.4974781168782556 2215 9 0.4547971287291688 2215 10 0.46988330774427567 2215 11 0.41866148001950165 2215 12 0.41141747062807354 2215 13 0.3876258681782119 2215 14 0.44996844366653477 2215 15 0.5293036164114184 2215 16 0.5412959675826343 2215 17 0.5276016992289456 2215 18 0.5312006220248833 2215 19 0.4788683488737604 2215 20 0.44438405238661005 2215 21 0.5574129902572409 2215 22 0.49122768097214475 2215 23 0.44760112409989006 2215 24 0.5135082394020216 2215 25 0.513176176453932 2216 1 0.6832863500375943 2216 2 0.6174548193854434 2216 3 0.7451322031488736 2216 4 0.80977384617632 2216 5 0.7028735092009413 2216 6 0.7667307545748656 2216 7 0.7021030868258222 2216 8 0.65229446908711 2216 9 0.6546520270020721 2216 10 0.539698475665773 2216 11 0.5683111235824754 2216 12 0.5294299915898009 2216 13 0.5287235450324701 2216 14 0.605447294873521 2216 15 0.49328593649482855 2216 16 0.5745421620698821 2216 17 0.5641747127889118 2216 18 0.6208307701448703 2216 19 0.6272634721890071 2216 20 0.6470746700067522 2216 21 0.6649211027480533 2216 22 0.5894168966278528 2216 23 0.5762193423407922 2216 24 0.6567876657596727 2216 25 0.5121550856918162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1265.0 3 862.0 4 133.0 5 224.0 6 196.0 7 481.0 8 575.0 9 1778.0 10 3429.0 11 5340.0 12 7387.0 13 10421.0 14 13650.0 15 16369.0 16 18935.0 17 19503.0 18 20063.0 19 19377.0 20 19321.0 21 19142.0 22 19434.0 23 19703.0 24 20261.0 25 21243.0 26 23341.0 27 25675.0 28 29133.0 29 35582.0 30 43642.0 31 56535.0 32 74379.0 33 101226.0 34 142777.0 35 214006.0 36 343237.0 37 673603.0 38 2139595.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.74242681625756 21.71722834636575 14.036995740663121 23.503349096713556 2 14.156647367150294 22.02104798393962 40.999416108667276 22.82288854024281 3 16.976438015757996 27.93202500738085 30.553212809887555 24.538324166973602 4 11.445950473330045 18.69644657939946 40.270440640316544 29.58716230695395 5 12.415198440218807 38.41206464204195 36.50985491995733 12.662881997781911 6 28.645621675901246 39.877594774837775 18.8884795676285 12.588303981632484 7 24.458416651998736 33.118243733907356 25.55444784612233 16.868891767971583 8 23.321158790728365 37.091682289647665 22.623847995529054 16.96331092409492 9 24.136252748825022 17.50389254062971 22.79084642769974 35.56900828284553 10 13.692421930343876 30.48704654241825 35.393509156078714 20.427022371159158 11 31.086892433708563 26.128999524368542 24.87865145736077 17.90545658456213 12 20.619661870576255 27.55870709958288 32.90086439281611 18.92076663702475 13 25.80896185523105 24.411132328968 27.634619366316322 22.145286449484626 14 20.138913267933905 23.638891867807814 29.326540436379826 26.89565442787845 15 22.075266498014233 31.128997470213516 25.16208210492855 21.633653926843703 16 21.115187411917155 31.355268090451716 26.768479873769063 20.76106462386206 17 20.04978406145309 30.25869098844039 29.191322827105598 20.500202123000925 18 20.275122004710415 30.09922456609993 29.801469106488337 19.824184322701317 19 20.808397056701775 29.334102823065457 29.869811955946712 19.98768816428606 20 21.80190254935842 29.05068534825359 29.16910467628884 19.97830742609915 21 21.95536645745846 29.15243981917254 28.457671050435547 20.434522672933454 22 21.012856219041748 29.021665755620013 29.212300163940302 20.75317786139794 23 20.891611566044148 29.31616866670723 28.976245545032597 20.81597422221602 24 20.98198458652964 29.02960846745118 29.117710867309558 20.87069607870962 25 21.44522822874102 28.884674678743306 28.402795648754537 21.267301443761138 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4691.0 1 4691.0 2 5194.0 3 5697.0 4 5697.0 5 5697.0 6 6844.5 7 7992.0 8 7992.0 9 7992.0 10 12030.5 11 16069.0 12 16069.0 13 16069.0 14 28191.0 15 40313.0 16 40313.0 17 40313.0 18 68075.5 19 95838.0 20 95838.0 21 95838.0 22 139024.0 23 182210.0 24 182210.0 25 182210.0 26 236058.5 27 289907.0 28 289907.0 29 289907.0 30 343048.5 31 396190.0 32 396190.0 33 396190.0 34 435431.0 35 474672.0 36 474672.0 37 474672.0 38 492544.5 39 510417.0 40 510417.0 41 510417.0 42 499210.0 43 488003.0 44 488003.0 45 488003.0 46 482075.0 47 476147.0 48 476147.0 49 476147.0 50 437601.0 51 399055.0 52 399055.0 53 399055.0 54 351461.0 55 303867.0 56 303867.0 57 303867.0 58 259386.0 59 214905.0 60 214905.0 61 214905.0 62 174668.5 63 134432.0 64 134432.0 65 134432.0 66 102325.0 67 70218.0 68 70218.0 69 70218.0 70 50762.5 71 31307.0 72 31307.0 73 31307.0 74 22050.0 75 12793.0 76 12793.0 77 12793.0 78 8759.5 79 4726.0 80 4726.0 81 4726.0 82 3186.5 83 1647.0 84 1647.0 85 1647.0 86 1091.5 87 536.0 88 536.0 89 536.0 90 335.0 91 134.0 92 134.0 93 134.0 94 86.5 95 39.0 96 39.0 97 39.0 98 28.5 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1388093631084263 2 0.08445818094618633 3 0.0579313440288066 4 0.09471810790607867 5 0.07907592418034116 6 0.07633673993343783 7 0.07427033778226512 8 0.08215149947510983 9 0.07756216446494721 10 0.07604840474955327 11 0.07547173438178414 12 0.07922009177228344 13 0.08111829839952348 14 0.07133893007943874 15 0.07145906973939065 16 0.07439047744221702 17 0.08323275641467694 18 0.08114232633151386 19 0.07744202480499532 20 0.0893358511402335 21 0.08662069482532053 22 0.0865486110293494 23 0.0918828119312138 24 0.09998022501197192 25 0.09450185651816524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4161823.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.7162286573315 #Duplication Level Percentage of deduplicated Percentage of total 1 88.00673156712773 71.03571468556676 2 9.109481276381446 14.705659473081697 3 1.7384246867141933 4.209572535491181 4 0.4940621629452734 1.5951533806090583 5 0.21419618559725645 0.8644554147098183 6 0.10961592134316905 0.5308670262971578 7 0.07047768013973138 0.398208478177778 8 0.043832212906069555 0.2830376735586518 9 0.032456732994236015 0.2357806573649439 >10 0.16226464160511714 2.315959945489095 >50 0.01032903475894459 0.5733054637463091 >100 0.0065484670773542815 0.9823913424073458 >500 8.344162451951702E-4 0.5034722035478136 >1k 6.854130312178009E-4 1.2757222934173722 >5k 2.980056657468064E-5 0.2341646528097314 >10k+ 2.980056657468064E-5 0.2565347737252989 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 10665 0.25625789467740456 No Hit CCCATGTACTCTGCGTTGATACCAC 9735 0.233911917926351 No Hit GTATCAACGCAGAGTACTTTTTTTT 4824 0.11591074392159398 No Hit GAGTACATGGGAAGCAGTGGTATCA 4643 0.11156168823133515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 2.4027931990380177E-5 0.0 0.0 2 0.0 0.0 2.4027931990380177E-5 0.0 0.0 3 0.0 0.0 2.4027931990380177E-5 2.4027931990380177E-5 0.0 4 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 5 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 6 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 7 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 8 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 9 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 10 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 11 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 12 0.0 0.0 2.4027931990380177E-5 4.8055863980760355E-5 0.0 13 0.0 0.0 2.4027931990380177E-5 7.208379597114053E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCGA 120 1.5290098E-6 10.289843 11 CAATCCG 120 1.5290098E-6 10.289843 10 ACAGCGC 85 6.610114E-4 10.056799 8 GAGTACG 80 0.004467165 9.513075 1 TAACCCG 100 2.7572448E-4 9.50003 5 GTCTAGG 355 0.0 9.111114 1 GTCCTAA 400 0.0 9.03742 1 GTATTAG 760 0.0 9.012387 1 ATAGGCG 130 4.3721662E-5 8.7692585 5 CCTACAC 525 0.0 8.68616 3 TTAGACA 635 0.0 8.677714 4 CCAGTAC 450 0.0 8.655999 3 TCTGTCG 310 0.0 8.5789175 8 CCTAGAC 355 0.0 8.56382 3 TAGGACA 495 0.0 8.44498 4 GTCTTAG 565 0.0 8.418651 1 GTGTTAG 605 0.0 8.333768 1 TAGACTG 605 0.0 8.322341 5 CTGTCGC 310 0.0 8.2724285 9 ATAGGAC 345 0.0 8.261293 3 >>END_MODULE