FastQCFastQC Report
Wed 25 May 2016
SRR1294943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161823
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT124200.2984269153205218No Hit
CCCATGTACTCTGCGTTGATACCAC108190.25995819620392313No Hit
GTACATGGGAAGCAGTGGTATCAAC103110.24775200675281003No Hit
GGTATCAACGCAGAGTACTTTTTTT70010.16821955186465162No Hit
TATCAACGCAGAGTACTTTTTTTTT66380.1594974125521436No Hit
GAGTACATGGGAAGCAGTGGTATCA45030.10819777775268194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC1106.847781E-59.4992797
GTATAAG7750.08.9526561
ATAACGC850.0074342328.94189553
GACCACG850.00744376248.9404987
GTATTAG6650.08.8613681
TAACCCG1000.002904418.5506865
GTCTAGA3700.08.47701551
GTCTTGC7150.08.3746221
CTTAGAC3750.08.3606723
CAGGACG1252.7427933E-48.3606714
CCTGGAC6650.08.286383
ACACCGT1502.5855883E-58.23399456
TAGACAG6500.08.1852735
GTCTTAT5700.08.1705631
AGACCGT1050.00451187568.1435116
GTCTAGT4000.08.0788521
TAGACTG6850.08.0444425
TAGGCCT4500.08.0228664
CTTATAC5100.08.01044753
CCAGTAC5100.08.01044753
Produced by FastQC (version 0.11.5)