FastQCFastQC Report
Wed 25 May 2016
SRR1294942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027929
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72600.3580006992355255No Hit
GTACATGGGAAGCAGTGGTATCAAC63150.31140143466561204No Hit
CCCATGTACTCTGCGTTGATACCAC59660.2941917591789456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52510.2589341145572651No Hit
GGTATCAACGCAGAGTACTTTTTTT48950.2413792593330437No Hit
TATCAACGCAGAGTACTTTTTTTTT41050.20242326038041766No Hit
GAGTACATGGGAAGCAGTGGTATCA28220.13915674562570976No Hit
GTGGTATCAACGCAGAGTACATGGG24210.1193828778029211No Hit
GTATCAACGCAGAGTACATGGGAAG23000.11341619948232902No Hit
GGTATCAACGCAGAGTACATGGGAA22200.10947128819598713No Hit
CATGTACTCTGCGTTGATACCACTG22160.10927404263167005No Hit
GCGTTGATACCACTGCTTCCCATGT21360.10532913134532816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC1500.013.3025413
GGACCGT751.4814601E-512.6647096
GGCTTAT1502.3926987E-810.1475421
ACACGGC750.002651562410.1317676
TCTAACC1151.0243046E-59.9149363
AGCGCAC2600.09.86361610
CCCTTAC1401.0386575E-69.5133211
CTATACG800.00450933869.5018154
CCCCGAT800.00450933869.5018152
TTAGATA2600.09.5018144
GTATAGA2151.03682396E-109.2920821
AGAGCGC2900.09.1709968
TTGGGCC1252.7480943E-59.1217414
GTCTAGC950.00180084639.012621
GGCATAT850.0073513978.9537141
GTATAAA3550.08.8433691
TGGACAG2151.0422809E-98.8367165
GTATTAG4100.08.81722451
AGGGTGC1304.3768123E-58.7678766
CTAGGAC1856.553273E-88.7313973
Produced by FastQC (version 0.11.5)