FastQCFastQC Report
Wed 25 May 2016
SRR1294942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027929
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT203071.0013664186468068No Hit
GGTATCAACGCAGAGTACTTTTTTT139390.6873514802539931No Hit
TATCAACGCAGAGTACTTTTTTTTT115930.5716669567820175No Hit
CCCATGTACTCTGCGTTGATACCAC66810.32944940380062615No Hit
GTACATGGGAAGCAGTGGTATCAAC61970.3055826905182578No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60800.2998132577619828No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39610.19532242006500225No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31640.15602124137482132No Hit
GAGTACATGGGAAGCAGTGGTATCA29020.14310165691205165No Hit
GCAGAGTACTTTTTTTTTTTTTTTT24790.12224293848551897No Hit
GTGGTATCAACGCAGAGTACATGGG23300.11489554121470723No Hit
CATGTACTCTGCGTTGATACCACTG22800.11242997166074356No Hit
GTATCAACGCAGAGTACATGGGAAG22080.10887955150303584No Hit
GGTATCAACGCAGAGTACATGGGAA21890.10794263507252966No Hit
GCGTTGATACCACTGCTTCCCATGT21330.10518119717209036No Hit
GTGGTATCAACGCAGAGTACTTTTT21100.10404703517726706No Hit
ATCAACGCAGAGTACTTTTTTTTTT20390.10054592641063863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGA550.0030691812.0908156
GGACCGT1103.8164217E-812.0908156
CGGTATC658.0141803E-411.6942362
ACCGGGC700.001492452810.8565238
ATACCGT803.772014E-410.6874176
ACCGTTA803.7737333E-410.686898
GGGTAAC1002.3839431E-510.4561061
AGCGCAC2300.010.32549710
GCGTGGT856.598255E-410.058248511
CGGTACT951.6445134E-49.9999234
TGCGTTA3550.09.90084412
GTCTATA1554.0274244E-89.8121821
TAGACCC1002.7559823E-49.4999274
AATCCTG2201.6370905E-119.4999265
CAGCGCA900.00111440329.4994579
GCACGGA3300.09.49945713
GGTATCA49150.09.2831431
ATCCTGC1856.697519E-99.2431726
CTAGGAC1651.0653821E-79.2122763
GTATTAG3850.09.1352051
Produced by FastQC (version 0.11.5)