FastQCFastQC Report
Wed 25 May 2016
SRR1294941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976674
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80180.20162578074038756No Hit
CCCATGTACTCTGCGTTGATACCAC70820.17808852322317595No Hit
GTATCAACGCAGAGTACTTTTTTTT39980.10053627730108125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC957.420931E-812.9969266
TGTGCGA701.0938806E-412.21170410
ACCGTCC658.04419E-411.6895438
CACCGTT856.612118E-410.0564457
GTACCGT1054.1192718E-59.95002656
TGGACCG1356.4470805E-79.852255
TTATACT7500.09.7544644
TTAGACT3300.09.50110154
ACACCGT1752.6839189E-89.2263896
ATTAGAC3000.09.1845143
GGAACGA1252.7595734E-59.11910515
ATCGACC950.00182868958.9978728
GTATAGG5350.08.8917371
GTCTTAG4200.08.8345751
CTAGACT4200.08.8224514
GTCCTAG4150.08.7117591
CCGTACC1554.2044685E-68.581644
CTAGTAC2554.0017767E-118.5697293
TAGACTG5000.08.5503455
AACACCG1000.00290539868.5503455
Produced by FastQC (version 0.11.5)