FastQCFastQC Report
Wed 25 May 2016
SRR1294941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976674
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110370.27754349489045366No Hit
CCCATGTACTCTGCGTTGATACCAC78700.19790407762869172No Hit
GTACATGGGAAGCAGTGGTATCAAC75310.1893793657714965No Hit
TATCAACGCAGAGTACTTTTTTTTT61060.15354540000010058No Hit
GGTATCAACGCAGAGTACTTTTTTT59310.1491447375369467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCG250.006036441318.99850512
AACCGTA600.00588183711.082467
GGACCGA909.518185E-510.5560516
TACACCG909.518185E-510.5560515
AATACCG750.002648092310.1338095
GCTCGTT951.646594E-49.99921211
ACACCGC1151.0268559E-59.9135096
CTCCGTA800.0045106939.5018812
GCACCGT1502.6498128E-79.5004456
TAGACAG6100.09.1889565
ACCGCTC1252.7589487E-59.1192828
TTACCGT950.00182397959.0004236
CAGCGTA950.00182606768.999299
TTCTACG850.00742694648.9429462
TAGGCCG850.0074361458.9415975
CCAGGAC6950.08.7486123
ACACCGT1107.1451603E-48.6367696
ATGCGCC1000.00290853658.54932711
CTAGGAC3600.08.4448413
TAGGACC2606.002665E-118.4042424
Produced by FastQC (version 0.11.5)