FastQCFastQC Report
Wed 25 May 2016
SRR1294940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341595
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC85830.6397608816371558No Hit
CCCATGTACTCTGCGTTGATACCAC81240.6055478739858154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA74020.5517313347172582No Hit
GAGTACATGGGAAGCAGTGGTATCA40720.30351931842322016No Hit
GTATCAACGCAGAGTACTTTTTTTT37770.2815305662290035No Hit
CATGTACTCTGCGTTGATACCACTG29670.22115467037369696No Hit
GCGTTGATACCACTGCTTCCCATGT28460.21213555506691661No Hit
GGTATCAACGCAGAGTACTTTTTTT27660.20617250362441722No Hit
ACGCAGAGTACATGGGAAGCAGTGG25780.19215933273454358No Hit
GTGGTATCAACGCAGAGTACATGGG25520.19022134101573127No Hit
GTATCAACGCAGAGTACATGGGAAG25360.1890287307272314No Hit
GGTATCAACGCAGAGTACATGGGAA24630.18358744628595067No Hit
TATCAACGCAGAGTACATGGGAAGC23960.17859339070285743No Hit
ACTCTGCGTTGATACCACTGCTTCC21560.1607042363753592No Hit
TATCAACGCAGAGTACTTTTTTTTT20660.15399580350254732No Hit
GCTTCCCATGTACTCTGCGTTGATA15580.11613042684267608No Hit
GGTATCAACGCAGAGTACATGGGGG15300.11404335883780127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTT350.002172724816.28264411
TACTTAC456.769224E-414.7755437
GCTATAA550.003039927712.1057921
GTCTTAG802.8298162E-511.8896171
AGCGCAA1104.977737E-711.22515710
CCTAGTC1053.456791E-610.8591472
GTCTACC1002.3653625E-510.4628631
CTAGTCC1106.0287475E-610.365553
GCAAGTA1201.5263231E-610.28972713
TCTACTC856.5805006E-410.060683
AGACCCG951.6403792E-410.0018475
TCTAATA1451.6110425E-79.8294012
AAGTGGC1451.618937E-79.82617
GTATAAA1856.330083E-109.7687661
GCATAAG800.00446917639.5116941
CCCCTAT1502.5983718E-79.5116941
GTCCTAA1304.1693165E-69.5116941
CCCTTAA900.00110084479.5116941
TTACACC1502.636716E-79.5021094
TTGGGCT1804.1582098E-99.5021094
Produced by FastQC (version 0.11.5)