FastQCFastQC Report
Wed 25 May 2016
SRR1294940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341595
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118970.8867802876426939No Hit
CCCATGTACTCTGCGTTGATACCAC89660.6683089904181218No Hit
GGTATCAACGCAGAGTACTTTTTTT87440.6517615226651858No Hit
GTACATGGGAAGCAGTGGTATCAAC82500.614939680007752No Hit
TATCAACGCAGAGTACTTTTTTTTT66940.4989583294511384No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39390.29360574540006484No Hit
GAGTACATGGGAAGCAGTGGTATCA39030.2909223722509401No Hit
CATGTACTCTGCGTTGATACCACTG33810.25201346158863147No Hit
GCGTTGATACCACTGCTTCCCATGT29590.22055836522944702No Hit
ACGCAGAGTACATGGGAAGCAGTGG27900.20796141905716706No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26280.1958862398861057No Hit
GTATCAACGCAGAGTACATGGGAAG25320.18873057815510644No Hit
GGTATCAACGCAGAGTACATGGGAA23800.17740078041435753No Hit
ACTCTGCGTTGATACCACTGCTTCC23760.17710262784223257No Hit
TATCAACGCAGAGTACATGGGAAGC23670.1764317845549514No Hit
GTGGTATCAACGCAGAGTACATGGG23570.17568640312463896No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20790.15496479936195348No Hit
GCTTCCCATGTACTCTGCGTTGATA16520.12313701228761288No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16130.12023002470939442No Hit
GTGGTATCAACGCAGAGTACTTTTT15980.11911195256392579No Hit
GGTATCAACGCAGAGTACATGGGGG14580.1086766125395518No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13480.1004774168061151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA400.00526439914.255791
AGCGTTA400.005280046714.249413510
CCGGATA655.4516044E-513.1537944
TGGGTTA701.0907258E-412.2142385
ATCGCTA550.003069016812.09041110
GATAGGC802.8668832E-511.8745127
TAGGACC1001.9288327E-611.3999554
GTATTGG1354.6929927E-911.2638331
GTCCTAA1104.9396294E-711.2318331
TAGATTG951.3597586E-510.9999575
GCTTATA803.755825E-410.6918421
CTTAAGC803.7700852E-410.6874583
CGGATAG803.7700852E-410.6874585
TAGGACG803.7700852E-410.6874584
TTAGACA909.512304E-510.5555144
CTAAACA1201.522716E-610.2916264
GCTCGCA750.002648071910.13291612
GGTCTAG856.5671233E-410.0629091
GTCTAAT951.6367197E-410.0040631
ATGACGA951.6441254E-49.99958811
Produced by FastQC (version 0.11.5)