FastQCFastQC Report
Wed 25 May 2016
SRR1294939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978153
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC260012.6581731078880297No Hit
CCCATGTACTCTGCGTTGATACCAC243272.487034236975197No Hit
GAGTACATGGGAAGCAGTGGTATCA120291.229766713387374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA110801.1327471264720346No Hit
CATGTACTCTGCGTTGATACCACTG93300.9538385099263613No Hit
GCGTTGATACCACTGCTTCCCATGT82990.8484357764071674No Hit
GTATCAACGCAGAGTACATGGGAAG74380.7604127370666961No Hit
ACGCAGAGTACATGGGAAGCAGTGG72380.7399660380329048No Hit
TATCAACGCAGAGTACATGGGAAGC71130.7271868511367854No Hit
GGTATCAACGCAGAGTACATGGGAA69660.7121585273469488No Hit
GTATCAACGCAGAGTACTTTTTTTT62530.639266045291483No Hit
ACTCTGCGTTGATACCACTGCTTCC62260.6365057409219211No Hit
GTGGTATCAACGCAGAGTACATGGG51300.5244578302167452No Hit
GGTATCAACGCAGAGTACTTTTTTT45470.46485570253324376No Hit
GCTTCCCATGTACTCTGCGTTGATA45430.464446768552568No Hit
GTACTCTGCGTTGATACCACTGCTT41730.42662037534005415No Hit
ATACCACTGCTTCCCATGTACTCTG35860.3666093136758769No Hit
TATCAACGCAGAGTACTTTTTTTTT34830.3560792636734744No Hit
GGGAAGCAGTGGTATCAACGCAGAG32850.33583703163002104No Hit
GATACCACTGCTTCCCATGTACTCT32290.33011195590055953No Hit
CAGTGGTATCAACGCAGAGTACATG32030.3274538850261667No Hit
CTGCTTCCCATGTACTCTGCGTTGA30540.3122210942459922No Hit
GCAGTGGTATCAACGCAGAGTACAT30120.307927287448896No Hit
CATGGGAAGCAGTGGTATCAACGCA28760.294023532105918No Hit
TACCACTGCTTCCCATGTACTCTGC28240.2887073903571323No Hit
GCAGAGTACATGGGAAGCAGTGGTA27660.28277784763733277No Hit
GTTGATACCACTGCTTCCCATGTAC26620.2721455641397614No Hit
ACATGGGAAGCAGTGGTATCAACGC25930.26509145297310344No Hit
CCATGTACTCTGCGTTGATACCACT23600.2412710485987366No Hit
CTTCCCATGTACTCTGCGTTGATAC23380.23902191170501957No Hit
CCACTGCTTCCCATGTACTCTGCGT23230.23748840927748524No Hit
AAGCAGTGGTATCAACGCAGAGTAC20650.2111121675238945No Hit
ATGGGAAGCAGTGGTATCAACGCAG19300.19731064567608544No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA17870.18269125586692472No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17830.18228232188624888No Hit
GTACATGGTAAGCAGTGGTATCAAC17370.17757958110847688No Hit
TGATACCACTGCTTCCCATGTACTC17090.17471704324374612No Hit
TTCCCATGTACTCTGCGTTGATACC16440.16807186605776397No Hit
ATCAACGCAGAGTACATGGGAAGCA15700.16050658741526122No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13640.13944648741045623No Hit
ACCATGTACTCTGCGTTGATACCAC13360.13658394954572547No Hit
GGAAGCAGTGGTATCAACGCAGAGT11170.11419481410372405No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9890.10110892672209766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC500.001496269313.3030443
CCTGTAC500.001496269313.3030443
GGACCTT500.001500892513.2976016
GGTGTCC604.0958408E-412.6643828
CGAACTC701.0914182E-412.21270715
CTTACAC550.003061789312.0936783
CTAAGAT1302.5993359E-911.6949843
TGCCCTA752.0757894E-411.397943510
GCCCTAA752.0757894E-411.397943511
GTACAAA1803.6379788E-1211.0983681
TTGGGCA600.005863013711.0858714
AGGTGTC600.00588090711.0813347
TCCTAAG1303.1943273E-810.9764071
GTCTTAC700.001473781310.8718691
CCTAAGA1408.300049E-910.8596282
AGATTAT1408.352799E-910.8551846
AGGATGC1158.822408E-710.7371936
GTTCTAA909.3760944E-510.5698741
TAGGGTG750.002644412710.1340975
TTAAGCA856.5744267E-410.0611264
Produced by FastQC (version 0.11.5)