FastQCFastQC Report
Wed 25 May 2016
SRR1294939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978153
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC265612.715423865182645No Hit
GTACATGGGAAGCAGTGGTATCAAC252662.583031488938847No Hit
GTATCAACGCAGAGTACTTTTTTTT186571.9073703193672156No Hit
GGTATCAACGCAGAGTACTTTTTTT139631.4274862930441352No Hit
GAGTACATGGGAAGCAGTGGTATCA118991.2164763590154095No Hit
TATCAACGCAGAGTACTTTTTTTTT115481.180592402211106No Hit
CATGTACTCTGCGTTGATACCACTG98691.0089423638224286No Hit
GCGTTGATACCACTGCTTCCCATGT86740.8867733370955259No Hit
ACGCAGAGTACATGGGAAGCAGTGG81260.830749381742938No Hit
GTATCAACGCAGAGTACATGGGAAG75360.7704316195932539No Hit
TATCAACGCAGAGTACATGGGAAGC74540.7620484729893995No Hit
GGTATCAACGCAGAGTACATGGGAA70470.7204394404556342No Hit
ACTCTGCGTTGATACCACTGCTTCC68250.697743604528126No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65420.6688115253953113No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51800.5295695049751931No Hit
GTGGTATCAACGCAGAGTACATGGG49220.5031932632216024No Hit
GCTTCCCATGTACTCTGCGTTGATA49220.5031932632216024No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39600.4048446408690665No Hit
GTACTCTGCGTTGATACCACTGCTT38460.39319002241980544No Hit
ATACCACTGCTTCCCATGTACTCTG37170.38000190154301017No Hit
CAGTGGTATCAACGCAGAGTACATG34350.3511720559053645No Hit
GATACCACTGCTTCCCATGTACTCT34040.34800281755512685No Hit
GGGAAGCAGTGGTATCAACGCAGAG33740.34493581270005813No Hit
CATGGGAAGCAGTGGTATCAACGCA33000.3373705340575554No Hit
CTGCTTCCCATGTACTCTGCGTTGA31640.32346677871457735No Hit
GCAGTGGTATCAACGCAGAGTACAT30960.3165149010430883No Hit
GCAGAGTACATGGGAAGCAGTGGTA30330.31007419084744414No Hit
TACCACTGCTTCCCATGTACTCTGC29050.2969883034658177No Hit
ACATGGGAAGCAGTGGTATCAACGC28610.29249002967838367No Hit
GCAGAGTACTTTTTTTTTTTTTTTT27530.2814488122001364No Hit
CCATGTACTCTGCGTTGATACCACT26860.27459916802381634No Hit
GTTGATACCACTGCTTCCCATGTAC25400.2596730777291487No Hit
CCACTGCTTCCCATGTACTCTGCGT24980.25537927093205254No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24890.25445916947553193No Hit
CTTCCCATGTACTCTGCGTTGATAC24560.2510854641349564No Hit
GTGGTATCAACGCAGAGTACTTTTT24430.24975642869775996No Hit
AAGCAGTGGTATCAACGCAGAGTAC20960.21428140587413216No Hit
ATGGGAAGCAGTGGTATCAACGCAG20380.2083518631543327No Hit
ATCAACGCAGAGTACTTTTTTTTTT18950.19373247334517196No Hit
ATCAACGCAGAGTACATGGGAAGCA17790.18187338790557306No Hit
GTACATGGTAAGCAGTGGTATCAAC17680.18074881945871454No Hit
TTCCCATGTACTCTGCGTTGATACC17370.17757958110847688No Hit
TGATACCACTGCTTCCCATGTACTC17230.17614831217611152No Hit
ACCATGTACTCTGCGTTGATACCAC14560.1488519689660002No Hit
GGAAGCAGTGGTATCAACGCAGAGT11030.11276354517135867No Hit
AAAAAGTACTCTGCGTTGATACCAC9920.10141562720760453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA250.006031451718.99948916
ACCGTGG453.5390913E-516.8875718
GTCTAGA400.00526031914.2569051
CCCTTAG400.00526031914.2569051
GTCCCGC500.00149973613.29896212
AGACAGC701.0898568E-412.21458156
AGGATGC701.0898568E-412.21458156
TAGACAG1251.4060788E-912.16029555
GTTCTAG550.003055940112.0967671
CGCAGAA550.003062971412.0930562
ATTAGAC658.01369E-411.6931893
GTCCAAT752.0630122E-411.4055241
TTATGAC600.00586972811.0841683
GGACTTC600.005871962311.0836026
TGTACAG700.001490244910.8574065
TTAGACA1106.0349066E-610.3638874
TGCCCTA750.002647783810.13254310
AGTGGTC750.002647783810.1325438
GCCCTAA750.002647783810.13254311
TAGGATG856.586825E-410.0590675
Produced by FastQC (version 0.11.5)