FastQCFastQC Report
Wed 25 May 2016
SRR1294938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4170428
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC84260.20204161299511705No Hit
CCCATGTACTCTGCGTTGATACCAC77920.18683933639425018No Hit
GTATCAACGCAGAGTACTTTTTTTT44530.1067756115199687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGT550.00307353812.0890156
CGCGGGC600.005883223411.0821316
CTAGACG700.001490380510.858834
GTATTAG5650.010.44031051
GAGCGTT950.00182759988.9984829
GTATTAA11850.08.992281
AGTACGG850.00743990628.9410645
TAGGACG1401.0839925E-58.82284
GTACCGC1000.0029106488.5486616
GTATAGG3800.08.5126651
GTTATAC3250.08.4780373
CTTGGAC3850.08.3907133
GCTAGAC2053.8578946E-88.342563
TCAATAC3650.08.3298623
CTAGGAC4450.08.3269373
GTCTAAC2303.597961E-98.2731771
TTGTACG1150.00110951998.2621544
TAACGCT1150.00110951998.2621544
GTCCTAA4500.08.2456011
ATAGTAC5200.08.2222353
Produced by FastQC (version 0.11.5)