FastQCFastQC Report
Wed 25 May 2016
SRR1294938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4170428
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT121590.2915528094478552No Hit
CCCATGTACTCTGCGTTGATACCAC84760.20324053070811918No Hit
GTACATGGGAAGCAGTGGTATCAAC81570.19559143569916562No Hit
GGTATCAACGCAGAGTACTTTTTTT67690.16230947998622683No Hit
TATCAACGCAGAGTACTTTTTTTTT65580.1572500472373579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA604.097582E-412.66557114
CGACCGC550.003071923512.08986410
ATACCGA855.3238444E-511.1772476
TATACCG700.001491450410.8578975
TGTACCG1053.4672903E-610.8578965
ATCGACC856.6031475E-410.0579548
TGCACGG1201.7006212E-59.5006595
CGATCAA900.00111522269.49917910
GTCCTAC2750.09.3332921
TAGACCG1054.4923648E-49.0482475
ACACCGC1054.4923648E-49.0482476
TACGGGT1054.4923648E-49.0482474
CACACCG2106.675691E-109.0482475
AGTACCG1054.4923648E-49.0482475
TATACGG850.00743490138.9417975
TATACTG8000.08.9068685
TAAACCG1401.0851718E-58.8220415
GCACCGT1107.14375E-48.6369636
GAGCCGT1107.14375E-48.6369636
CCTACAC4300.08.6168783
Produced by FastQC (version 0.11.5)