FastQCFastQC Report
Wed 25 May 2016
SRR1294937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791820
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44370.1170150481826669No Hit
CCCATGTACTCTGCGTTGATACCAC39390.10388151336297609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG400.005278839314.2508615
ACGTCGC658.0435956E-411.6896196
AGTACGG1501.7644197E-1011.4029473
ACCGTCC752.0797529E-411.3973798
TCGACCG700.001495289210.8545049
TGTACGA750.002643400310.1359532
GTCTTAG3750.09.6418871
TACGACC1002.749309E-49.50270754
TAGGCCG1002.755513E-49.5005745
CGACCGC800.00452557079.49806710
GAACCGT800.0045265569.4978166
TAGACAG5450.09.4134145
GTATTAA7100.09.3810051
TAGGACC3100.09.1961684
TAGGACG1451.7020393E-69.1747852
TAGGACT4850.09.01287654
TACGTCG850.00743518968.9417175
GTACCGT850.00745291968.939126
CCGTCCA1304.384504E-58.7670999
GTGACCG1201.76961E-48.7062169
Produced by FastQC (version 0.11.5)