FastQCFastQC Report
Wed 25 May 2016
SRR1294937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791820
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87050.22957313374580018No Hit
GGTATCAACGCAGAGTACTTTTTTT51250.13515936937934817No Hit
TATCAACGCAGAGTACTTTTTTTTT48730.1285134842898661No Hit
GTACATGGGAAGCAGTGGTATCAAC43560.11487887083247623No Hit
CCCATGTACTCTGCGTTGATACCAC43340.11429867451514049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT751.4798385E-512.6667286
GTCTACG1104.9322625E-711.23473551
TACGCTG1054.108229E-59.9524295
TGGTCGG1151.0284566E-59.91217710
ACACCGT1502.651268E-79.5000476
TGAACCG1002.7570545E-49.5000465
GAACCGT1002.7570545E-49.5000466
GGTCGGC900.0011151849.49916911
TACATCG1252.7561216E-59.1200455
TACTCCG1701.6868216E-78.941225
ACTCCGT1701.6868216E-78.941226
TCTAGAC3200.08.9065283
GGTATCA31750.08.7727571
GTATCAA52650.08.7028371
TTAGACT2850.08.6668234
ATCGACC1000.00290870188.54925258
CTATTAG4250.08.49976351
TGCGTTG11200.08.48140112
GTTAGGC1701.6124613E-68.3826153
CTAATAC3550.08.2960333
Produced by FastQC (version 0.11.5)