FastQCFastQC Report
Wed 25 May 2016
SRR1294936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931806
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC247490.8441554454830913No Hit
CCCATGTACTCTGCGTTGATACCAC214290.7309146648857394No Hit
GAGTACATGGGAAGCAGTGGTATCA99290.33866497305756244No Hit
GTATCAACGCAGAGTACATGGGAAG70750.24131883214646538No Hit
GCGTTGATACCACTGCTTCCCATGT70410.24015913740540815No Hit
CATGTACTCTGCGTTGATACCACTG69120.2357591191231616No Hit
GTATCAACGCAGAGTACTTTTTTTT58280.19878532208474914No Hit
ACGCAGAGTACATGGGAAGCAGTGG51070.17419297184056518No Hit
TATCAACGCAGAGTACATGGGAAGC47260.1611975690069534No Hit
GCTTCCCATGTACTCTGCGTTGATA45060.1536936618589361No Hit
GGTATCAACGCAGAGTACATGGGAA43540.14850914419303324No Hit
ACTCTGCGTTGATACCACTGCTTCC39730.13551374135942146No Hit
GTGGTATCAACGCAGAGTACATGGG35770.12200670849299032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32990.11252449855140483No Hit
GGTATCAACGCAGAGTACTTTTTTT32300.11017100040043577No Hit
TATCAACGCAGAGTACTTTTTTTTT32180.10976169637418028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT604.1008115E-412.6641518
TACGACC600.00587002411.0852964
TTGTGCG803.7797506E-410.6853789
GTCCTAG2600.010.610351
GTCCTAC1752.3101165E-1010.3281041
CCGTTCT750.002653133410.1313229
TCTACGT856.587992E-410.0602622
AAGATCG856.5962697E-410.05888755
GTAGGAC1554.058529E-89.8078533
GTACTAG3300.09.5127281
AATACCG800.00451722939.500065
GCGATTC800.0045242329.49827613
AGAAGCG1054.4941856E-49.0476765
TTCGCTA1054.5033972E-49.0458238
TCCGTTC1606.7648216E-78.9044828
GTGTAGA3800.08.7617231
GTCTTAC2659.094947E-128.61531
TAGACAG4200.08.5952925
GTCTAAG2901.8189894E-128.5286521
GTTCTGG3800.08.5113871
Produced by FastQC (version 0.11.5)