FastQCFastQC Report
Wed 25 May 2016
SRR1294936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931806
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC241750.8245770695605371No Hit
CCCATGTACTCTGCGTTGATACCAC232720.7937769415848115No Hit
GTATCAACGCAGAGTACTTTTTTTT158200.539599141280153No Hit
GAGTACATGGGAAGCAGTGGTATCA96200.32812539438148364No Hit
GGTATCAACGCAGAGTACTTTTTTT91420.3118214506689733No Hit
TATCAACGCAGAGTACTTTTTTTTT90260.3078648450818369No Hit
CATGTACTCTGCGTTGATACCACTG72810.2483452179305179No Hit
GTATCAACGCAGAGTACATGGGAAG71160.24271728756950495No Hit
GCGTTGATACCACTGCTTCCCATGT70140.2392382033463333No Hit
ACGCAGAGTACATGGGAAGCAGTGG56730.1934984784122824No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51230.17473871054223916No Hit
TATCAACGCAGAGTACATGGGAAGC47110.16068593897413402No Hit
GCTTCCCATGTACTCTGCGTTGATA46180.15751383277065398No Hit
ACTCTGCGTTGATACCACTGCTTCC42340.14441610393047835No Hit
GGTATCAACGCAGAGTACATGGGAA41930.14301764850743875No Hit
GTGGTATCAACGCAGAGTACATGGG35490.12105166576506085No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34400.11733382085990683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA400.00528498414.2482110
TAGACAG4200.09.7276235
ATTAGAC3300.09.5017233
CTAGACA3100.09.5013994
TTAGACT3750.09.1213434
ATTGGAC1804.209869E-88.9738493
TTATACT5200.08.9532414
TATACGG850.00742906158.9424935
CCGTCCA850.007445718.9400539
TAGACTG4050.08.9148935
CTATACT4700.08.8949274
TGGACAG4400.08.8535775
CCCCTAG1401.0755584E-58.8279981
CGTTCCA1201.766999E-48.70723910
TATACTG5600.08.6530595
TGCGTTG62150.08.60471112
ATTATAC4800.08.511963
CCATGTA61600.08.499832
CTGCGTT62850.08.49376111
GTGTAGG3700.08.4792831
Produced by FastQC (version 0.11.5)