FastQCFastQC Report
Wed 25 May 2016
SRR1294935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428078
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC54940.10121446301987554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA1451.618373E-79.8281975
CCGGCGT800.0045237599.49866313
GTCTTAA5950.09.4315371
TACGACC1951.6825652E-99.2575844
GTATCAA58350.09.0305941
TACACCG2151.0422809E-98.8377585
CCGTGCA1951.6565536E-88.7678369
TCGCGTT1107.159574E-48.63514810
TTATACT8750.08.5782294
CCGTCTG2002.5498593E-88.5486399
GTCTAGG5800.08.527521
TACGGGT1451.6882912E-58.518324
ATAGGAC4950.08.4455143
ACTCGCG1252.7489275E-48.3590558
GTATTAA10000.08.2749741
GTGTAGA7300.08.2085251
TAGACTG6150.08.1875015
TTAGACA6750.08.1639984
ACCGTCT2355.5424607E-98.0841928
GTACGAC1304.1832408E-48.0394793
Produced by FastQC (version 0.11.5)