FastQCFastQC Report
Wed 25 May 2016
SRR1294935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428078
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132710.24448801214720936No Hit
TATCAACGCAGAGTACTTTTTTTTT72280.13315947191621047No Hit
GGTATCAACGCAGAGTACTTTTTTT67650.12462974924089153No Hit
GTACATGGGAAGCAGTGGTATCAAC56480.10405156300259503No Hit
CCCATGTACTCTGCGTTGATACCAC54810.10097496756678882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA750.002651041810.13257212
GTGCGAC750.002651041810.13257211
GTCTAGC2600.09.5061161
ACCGTTC1304.2480315E-69.4992878
TAGACCG850.00743758268.9414935
CAGCGTA850.0074443298.9405049
GTATCAA74350.08.9115841
CTAGGAC5550.08.90129853
GGTATCA42300.08.8543971
GTAGCCC5700.08.8337273
GTCCTAA4850.08.8201081
ACACCGT2502.5465852E-118.7403096
CCTACAC5950.08.4625623
CTATACT7000.08.2788654
AATTCCG1050.00451415178.1431465
TAAGACT6300.08.1431464
CGTGCAA1401.0409622E-48.14224510
GTATAAA9700.08.13411
TCTATAC6550.08.1224993
GTATTAA10550.08.0193781
Produced by FastQC (version 0.11.5)