FastQCFastQC Report
Wed 25 May 2016
SRR1294934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4982507
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC138890.2787552531285957No Hit
CCCATGTACTCTGCGTTGATACCAC125840.25256361907770525No Hit
GAGTACATGGGAAGCAGTGGTATCA58010.11642733266606549No Hit
GTATCAACGCAGAGTACTTTTTTTT54600.10958338844280599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA855.3371354E-511.174616
GTTCCGG856.5229624E-410.0714091
TAAACCG951.6457385E-49.9998425
CTAGGAC4300.09.9428013
TATACCG1151.0277894E-59.9128865
AGTCCGT1356.8695863E-69.14662556
GAACCGT1054.5033603E-49.0461136
CGTAGTA1201.7632429E-48.7092452
GTATACG1107.0609123E-48.647171
GTATAAT10700.08.4451331
GTCTAGG5300.08.4350691
CTAATAC7550.08.4312623
GTCTTAG4900.08.3471661
TGCGTTG36550.08.31607212
TAGGACT6400.08.313374
GTCCTAA5400.08.2788641
ATAATAC12400.08.2749773
CTGCGTT37300.08.17424211
GACTCGT1050.0045222318.1415016
CTAGAAC5850.08.1204273
Produced by FastQC (version 0.11.5)