FastQCFastQC Report
Wed 25 May 2016
SRR1294934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4982507
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT147570.29617620206052897No Hit
GTACATGGGAAGCAGTGGTATCAAC135570.2720919408643079No Hit
CCCATGTACTCTGCGTTGATACCAC135340.271630325858047No Hit
GGTATCAACGCAGAGTACTTTTTTT86480.17356724235410007No Hit
TATCAACGCAGAGTACTTTTTTTTT82100.16477648701747935No Hit
GAGTACATGGGAAGCAGTGGTATCA57120.11464108329401243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG1052.7263923E-711.7612157
GGACCGT909.522702E-510.5556786
AGCGTGC1201.5269143E-610.2910638
CAACGCG950.00182590178.99947315
AACGCGA850.0074431378.94065316
GTATACG1107.110739E-48.641061
CTAGGAC4400.08.6366383
AGTACCG1000.0029064238.5500995
GTCTTAG5350.08.3503331
CCGGAAT1606.4619708E-68.31201313
GTCATAG5150.08.3054851
AGCACCG1150.00111119558.2609655
GTCTATA5300.08.2497661
GTATTAT10200.08.2005361
CGTTCCG2252.1895175E-88.02175210
TACACAG11650.07.99150945
ATTAGAC4800.07.91691733
TGCGTTG39850.07.8665412
GTATTAC5450.07.84830241
GTATTAG8850.07.840421
Produced by FastQC (version 0.11.5)