FastQCFastQC Report
Wed 25 May 2016
SRR1294933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614873
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC258491.6006831496966016No Hit
CCCATGTACTCTGCGTTGATACCAC246441.5260642787389473No Hit
GAGTACATGGGAAGCAGTGGTATCA110890.6866793859331353No Hit
CATGTACTCTGCGTTGATACCACTG86800.5375035683920655No Hit
GCGTTGATACCACTGCTTCCCATGT74420.46084119308453353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA73450.454834528783378No Hit
GTATCAACGCAGAGTACATGGGAAG67800.41984725733850276No Hit
TATCAACGCAGAGTACATGGGAAGC64970.40232265942894585No Hit
ACGCAGAGTACATGGGAAGCAGTGG64460.3991645163427713No Hit
ACTCTGCGTTGATACCACTGCTTCC55750.3452283863808485No Hit
GGTATCAACGCAGAGTACATGGGAA55420.34318488203097086No Hit
GTATCAACGCAGAGTACTTTTTTTT44630.27636848222739496No Hit
GCTTCCCATGTACTCTGCGTTGATA39590.2451585976110815No Hit
GTGGTATCAACGCAGAGTACATGGG37900.2346933783647383No Hit
GTACTCTGCGTTGATACCACTGCTT35300.2185930410626718No Hit
ATACCACTGCTTCCCATGTACTCTG32310.20007765316529535No Hit
CAGTGGTATCAACGCAGAGTACATG28630.17728948344544743No Hit
GGTATCAACGCAGAGTACTTTTTTT28180.17450288660470514No Hit
GGGAAGCAGTGGTATCAACGCAGAG27880.17264515537754363No Hit
TATCAACGCAGAGTACTTTTTTTTT27100.16781505418692366No Hit
GCAGAGTACATGGGAAGCAGTGGTA25900.1603841292782776No Hit
CTGCTTCCCATGTACTCTGCGTTGA25750.15945526366469687No Hit
GATACCACTGCTTCCCATGTACTCT25650.15883601992230967No Hit
TACCACTGCTTCCCATGTACTCTGC25520.15803100305720635No Hit
GCAGTGGTATCAACGCAGAGTACAT25380.1571640618178643No Hit
CATGGGAAGCAGTGGTATCAACGCA24470.15152894376214104No Hit
GTTGATACCACTGCTTCCCATGTAC23480.14539843071250802No Hit
ACATGGGAAGCAGTGGTATCAACGC20950.12973156403011257No Hit
CCACTGCTTCCCATGTACTCTGCGT20700.12818345467414466No Hit
CCATGTACTCTGCGTTGATACCACT20520.12706881593784775No Hit
CTTCCCATGTACTCTGCGTTGATAC19290.11945211790648552No Hit
AAGCAGTGGTATCAACGCAGAGTAC17970.11127810050697487No Hit
GTACATGGTAAGCAGTGGTATCAAC17280.10700531868450336No Hit
ATGGGAAGCAGTGGTATCAACGCAG17170.10632415056787749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT400.00527396514.2521384
GAACGAT400.00528590514.2472816
CTAATAG1051.9894287E-812.6685683
AGACTAT751.4815701E-512.664256
TTAGACT658.013617E-411.6940634
TAACGGT658.013617E-411.6940634
ATAAGAC658.013617E-411.6940633
GTACCTA600.00588417611.0812186
TATTAGA1303.2581738E-810.9631832
CCGGTAC803.7656166E-410.6891043
ACTAACT909.533101E-510.5535418
GAACAAA5000.010.2707711
TATACAG951.6429642E-410.0005715
CTTGTAC1252.564304E-69.8814833
AAGGGCG1252.5673544E-69.8805645
GTATAAA1451.5918522E-79.8379041
GTTTTAG1502.606812E-79.5099741
TTTATAC1304.229041E-69.5014263
TAAGACT900.00111203489.5014264
TGGACCG900.00111298099.5005425
Produced by FastQC (version 0.11.5)