FastQCFastQC Report
Wed 25 May 2016
SRR1294933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614873
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC267721.6578393471189377No Hit
GTACATGGGAAGCAGTGGTATCAAC259081.6043366877766858No Hit
GTATCAACGCAGAGTACTTTTTTTT139900.8663219955996541No Hit
GAGTACATGGGAAGCAGTGGTATCA112170.694605705835691No Hit
CATGTACTCTGCGTTGATACCACTG92560.5731720079535666No Hit
GGTATCAACGCAGAGTACTTTTTTT92220.5710665792294503No Hit
TATCAACGCAGAGTACTTTTTTTTT89860.556452426909113No Hit
GCGTTGATACCACTGCTTCCCATGT80110.4960761620263637No Hit
ACGCAGAGTACATGGGAAGCAGTGG70330.4355141240208983No Hit
GTATCAACGCAGAGTACATGGGAAG68660.42517275352303247No Hit
TATCAACGCAGAGTACATGGGAAGC67270.41656526550385076No Hit
ACTCTGCGTTGATACCACTGCTTCC57390.35538398375599817No Hit
GGTATCAACGCAGAGTACATGGGAA54860.3397171170736027No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45030.2788454571969437No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44130.27327226351545914No Hit
GCTTCCCATGTACTCTGCGTTGATA41880.2593392793117477No Hit
GTGGTATCAACGCAGAGTACATGGG37340.23122561340737013No Hit
GTACTCTGCGTTGATACCACTGCTT34390.21295792300694855No Hit
ATACCACTGCTTCCCATGTACTCTG33130.20515545185287015No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30940.19159401389459108No Hit
GGGAAGCAGTGGTATCAACGCAGAG30710.1901697532871006No Hit
CAGTGGTATCAACGCAGAGTACATG29690.18385346711475145No Hit
GCAGAGTACATGGGAAGCAGTGGTA28680.177599105316641No Hit
GCAGTGGTATCAACGCAGAGTACAT27360.16942508791713032No Hit
GATACCACTGCTTCCCATGTACTCT26940.1668242641991042No Hit
TACCACTGCTTCCCATGTACTCTGC26740.16558577671432984No Hit
CTGCTTCCCATGTACTCTGCGTTGA26610.16478075984922652No Hit
CATGGGAAGCAGTGGTATCAACGCA26390.16341842361597475No Hit
CCATGTACTCTGCGTTGATACCACT23280.1441599432277337No Hit
ACATGGGAAGCAGTGGTATCAACGC22190.1374101864357135No Hit
GTTGATACCACTGCTTCCCATGTAC22060.1366051695706102No Hit
CCACTGCTTCCCATGTACTCTGCGT21580.13363279960715177No Hit
CTTCCCATGTACTCTGCGTTGATAC20700.12818345467414466No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19900.12322950473504728No Hit
AAGCAGTGGTATCAACGCAGAGTAC19250.11920442040953066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19220.1190186472868145No Hit
ATGGGAAGCAGTGGTATCAACGCAG17930.11103040301001998No Hit
GTACATGGTAAGCAGTGGTATCAAC17820.1103492348933941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG400.00527865114.2502325
CTCCGCG604.0937567E-412.6660899
TTAACAC1001.4422403E-712.3502013
TAACGGT550.003068160312.0911054
ATACCGT550.003068160312.0911056
GTAACCC550.003068160312.0911053
ACCGAAC550.003069583812.0903588
ACCGTCT855.3253993E-511.1759618
CTAGTAG600.005875115711.0835133
AGCGGTT600.005877824511.0828288
GCGACCT700.001492025110.856647513
CCGTCTG803.772437E-410.6870129
CGCAGAA750.002644589910.1347542
TCTATAG750.002647308610.1334993
TAGCCCG856.5922516E-410.0589885
CCTATAC856.5922516E-410.0589883
GAGTGTC856.596006E-410.0583657
GTCTTAG951.6374903E-410.003881
ATTAGAG951.6436432E-410.0001633
GTATTAC1054.0870174E-59.9562431
Produced by FastQC (version 0.11.5)