FastQCFastQC Report
Wed 25 May 2016
SRR1294932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5657598
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC158360.2799067731570889No Hit
CCCATGTACTCTGCGTTGATACCAC144890.25609808261385836No Hit
GAGTACATGGGAAGCAGTGGTATCA66470.11748802230204408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG9150.09.6555545
TACTCCG1304.2451156E-69.4998195
TATACTG12900.09.1316095
GGCCCGT1054.5057238E-49.0456876
GTCCTAA5550.08.91391
GTCTTAG6250.08.6766521
GTATTAA10250.08.63211
GTATTAG8450.08.5568561
ATACCGT1451.6955846E-58.5154226
GTACTAG4400.08.4327521
TATTCCG2053.869536E-88.3413055
TATACCG1150.0011115918.2607125
GGACCGT1150.00111373448.2591056
GTGCTAG6650.08.1547491
CTAATAC7350.08.1434223
AGTACCG1050.00451642378.1427025
TACACCG1050.00451642378.1427025
CTGATCG1050.0045247058.1410469
ATTATAC10000.08.0755613
ATCTGCG1304.1986507E-48.0367447
Produced by FastQC (version 0.11.5)