FastQCFastQC Report
Wed 25 May 2016
SRR1294932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5657598
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC154030.27225334850585No Hit
GTACATGGGAAGCAGTGGTATCAAC153340.271033749658424No Hit
GTATCAACGCAGAGTACTTTTTTTT144890.25609808261385836No Hit
GGTATCAACGCAGAGTACTTTTTTT86690.15322757113531218No Hit
TATCAACGCAGAGTACTTTTTTTTT79920.14126136215404486No Hit
GAGTACATGGGAAGCAGTGGTATCA65020.11492509718788788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT803.7738457E-410.6875466
AGCGTAG803.7760037E-410.68688410
GTGTTCG1002.4029749E-510.4493979
TAGACCG1106.842956E-59.500045
GGACCGT800.0045184049.500046
GTACCGT900.00111442859.5000396
ACGATTT950.0018259668.99948115
AGTGCGC850.0074433418.940668
CGAGGCG850.0074433418.9406612
AACAGCG1606.756418E-78.9057377
AGAGTCG1502.7051065E-68.8667045
ACAGCGC1502.707373E-68.8661558
AGAACCG1107.1493775E-48.63645
TTAGTAC4750.08.6001883
AACCGTG1554.2156425E-68.580157
TAGGACC3900.08.5256774
TATTCCG1951.5384467E-78.2820855
AGTACTG7400.08.2162515
CCGTGCA1856.132068E-78.2157439
GTATTAA10550.08.1988391
Produced by FastQC (version 0.11.5)