FastQCFastQC Report
Wed 25 May 2016
SRR1294931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5585845
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC75650.13543161330112097No Hit
CCCATGTACTCTGCGTTGATACCAC70070.1254420772506219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT604.048476E-412.6838271
GACTCGC658.041217E-411.6902126
AGCACCG1201.7023458E-59.55
GTCTCGT1606.768187E-78.90465459
GTATTAA11650.08.4105211
GACCGTG1701.6179783E-68.3808517
CTAGGAC5950.08.1434413
AGACCGT1050.00452292348.1413986
TAATACT10350.08.0778734
ATAATAC10850.07.9683133
TGTCGCC3700.07.958318710
CTAATAC7600.07.87556463
GTATCAA55600.07.81903271
GTATTAC6600.07.78325841
TACGCTG1100.00685750237.77272755
CGGTCAC1100.00686631357.771612610
GTCTTAT6500.07.7566491
TGCGTTG21950.07.74618112
CCAGGAC9100.07.72582863
GTCTAGG5550.07.7131381
Produced by FastQC (version 0.11.5)