FastQCFastQC Report
Wed 25 May 2016
SRR1294931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5585845
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT117080.20960123311692322No Hit
CCCATGTACTCTGCGTTGATACCAC76890.13765151020123184No Hit
GTACATGGGAAGCAGTGGTATCAAC74700.1337308858373263No Hit
GGTATCAACGCAGAGTACTTTTTTT69200.12388456894167311No Hit
TATCAACGCAGAGTACTTTTTTTTT66920.11980282302856596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1001.0082658E-813.3009275
AACCGAC701.0926271E-412.2133887
AGGACCG1201.700943E-59.5006625
TAGACTG8400.08.9351465
ATTAGAC4700.08.89423753
TTACCGT1000.0029050078.5505964
CTAGCAC6500.08.4775133
GTATCAA70250.08.2264671
TAGACAG8900.08.2196755
TAAGACA9550.08.1576374
TGAACCG1050.0045127988.1434255
GTATTAA9700.08.1332191
CTAGACT6550.08.1227044
TATACTG12750.08.1221355
GTATTAG9150.08.1026971
ATACCGT1659.746223E-68.0611686
CTACACT7900.08.0575234
TAGTACT5800.08.0264224
GTACTAG4150.08.0163381
AGACCGT1909.166579E-78.0005586
Produced by FastQC (version 0.11.5)