FastQCFastQC Report
Wed 25 May 2016
SRR1294930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641604
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC84160.18131663106115903No Hit
CCCATGTACTCTGCGTTGATACCAC76850.16556776493643147No Hit
GTATCAACGCAGAGTACTTTTTTTT48910.10537305638309515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTG855.3353702E-511.17493511
GTCCTAA4750.010.2123631
AACTCCG951.6477024E-49.9986277
TACACCG1451.6197737E-79.8275095
GTCTTAC5050.09.7940351
GTACGAC800.00451699239.5003343
TGTCGAG800.00452302269.49879710
GTATTAA9900.09.3193631
TAAGACG1356.8497884E-69.1486684
TATACCG1151.1116495E-49.0868845
ACACCGT1606.76293E-78.9050266
CTAGGAC4700.08.89392953
TAGACAG6250.08.6639315
TACGACC1000.00290515868.5504854
ATTATAC7850.08.2295893
GACTGTC4400.08.2034197
CTTAGAC3850.08.1431433
GTCCGCA1401.0416156E-48.1417411
TTATACT7600.08.1254614
GTCCTAT4100.08.1195851
Produced by FastQC (version 0.11.5)