FastQCFastQC Report
Wed 25 May 2016
SRR1294930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641604
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT143610.3093973548799079No Hit
GGTATCAACGCAGAGTACTTTTTTT85430.18405275417721975No Hit
GTACATGGGAAGCAGTGGTATCAAC83400.17967926604682347No Hit
CCCATGTACTCTGCGTTGATACCAC82580.17791263537346141No Hit
TATCAACGCAGAGTACTTTTTTTTT77220.16636490316709482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA700.001493378910.85626312
TAGCCGT750.002647498610.1341494
TAACGAT800.00451530389.5007654
CGTGCGG1151.1125357E-49.0862210
TTATACT8900.09.0737644
CCGTGCG950.00182622648.999279
GACGGCG950.00182622648.999277
AACACCG1252.742966E-48.3606735
CTGTTCG1252.7477552E-48.3593239
GTATTAA10500.08.2376361
TAGTACC3700.08.2168774
TCTATAC6500.08.1853633
GTATCAA81350.08.1437731
CTAGGAC3850.08.14363
TAGGACG1050.00451206568.1435134
TAGACAG6700.08.0827415
GTGTTAG6000.08.0792191
GTGTAAG6850.08.0479981
CCGTAGA1304.1651525E-48.0426621
TACGCTG1304.1849792E-48.0391085
Produced by FastQC (version 0.11.5)