FastQCFastQC Report
Wed 25 May 2016
SRR1294929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493333
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA113992.3106096693308578No Hit
GTACATGGGAAGCAGTGGTATCAAC108052.1902041825703935No Hit
CCCATGTACTCTGCGTTGATACCAC103382.095541956447268No Hit
GAGTACATGGGAAGCAGTGGTATCA50051.0145277125187246No Hit
CATGTACTCTGCGTTGATACCACTG41040.8318924539813878No Hit
GCGTTGATACCACTGCTTCCCATGT34660.7025680422757042No Hit
ACGCAGAGTACATGGGAAGCAGTGG32050.6496626011233791No Hit
GGTATCAACGCAGAGTACATGGGAA30760.6235139348067127No Hit
TATCAACGCAGAGTACATGGGAAGC30520.6186490666547748No Hit
GTATCAACGCAGAGTACATGGGAAG30160.6113517644268679No Hit
ACTCTGCGTTGATACCACTGCTTCC27470.5568247005572301No Hit
GTGGTATCAACGCAGAGTACATGGG19480.3948651316656295No Hit
GCTTCCCATGTACTCTGCGTTGATA19460.3944597259863013No Hit
GTACTCTGCGTTGATACCACTGCTT18120.3672975454713145No Hit
GTATCAACGCAGAGTACTTTTTTTT16760.3397299592769995No Hit
ATACCACTGCTTCCCATGTACTCTG15320.3105407503653719No Hit
GGGAAGCAGTGGTATCAACGCAGAG14230.28844614084198705No Hit
GATACCACTGCTTCCCATGTACTCT13710.27790559317945485No Hit
CAGTGGTATCAACGCAGAGTACATG13240.26837855971524305No Hit
CATGGGAAGCAGTGGTATCAACGCA12830.2600677432890157No Hit
GCAGTGGTATCAACGCAGAGTACAT12820.25986504044935166No Hit
GGTATCAACGCAGAGTACTTTTTTT12780.25905422909069536No Hit
CTGCTTCCCATGTACTCTGCGTTGA12720.25783801205271084No Hit
GCAGAGTACATGGGAAGCAGTGGTA12170.2466893558711864No Hit
TACCACTGCTTCCCATGTACTCTGC11710.2373650252466387No Hit
ACATGGGAAGCAGTGGTATCAACGC11440.2318920485757085No Hit
GTTGATACCACTGCTTCCCATGTAC11380.23067583153772403No Hit
TATCAACGCAGAGTACTTTTTTTTT10220.20716230213669062No Hit
CTTCCCATGTACTCTGCGTTGATAC10220.20716230213669062No Hit
CCATGTACTCTGCGTTGATACCACT10040.2035136510227372No Hit
CCACTGCTTCCCATGTACTCTGCGT9990.20250013682441675No Hit
AAGCAGTGGTATCAACGCAGAGTAC9030.183040664216665No Hit
ATGGGAAGCAGTGGTATCAACGCAG8990.1822298528580087No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7700.15608118654134226No Hit
TGATACCACTGCTTCCCATGTACTC7620.15445956382402962No Hit
TTCCCATGTACTCTGCGTTGATACC7110.14412171900116147No Hit
ATCAACGCAGAGTACATGGGAAGCA7010.14209469060452068No Hit
GTACATGGTAAGCAGTGGTATCAAC5650.1145271044102057No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5490.1112838589755804No Hit
AAACAAAAAAAAAAAAAAAAAAAAA5170.1047973681063298No Hit
GGAAGCAGTGGTATCAACGCAGAGT5060.1025676368700249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCC400.00527327414.249624
CCCTAGA500.001479100313.321261
GTGTTAA550.003026901512.1102351
GTACATA802.8107292E-511.8939821
GAACAAA2850.011.6853151
TAGACAG803.7617696E-410.6872165
GTGCTAA750.002607301610.1495311
TACATAG750.00264267710.1330632
TCTGGCC850.00742139578.9409383
AATCAAC1606.410528E-68.31227919
GTGCTGG1200.00165897277.92932131
AGTGCTG1100.00684310967.7717328
AAAAGTA1250.002518047.5997972
GAAGCAG29000.07.53351749
GAAAAAA8100.07.5181711
TAATCCC1409.216685E-47.4640865
CCATGTA29550.07.36190132
AGTGGTA31150.07.31848514
GGAAGCA30100.07.22664938
CAGTGGT31600.07.214265313
Produced by FastQC (version 0.11.5)