FastQCFastQC Report
Wed 25 May 2016
SRR1294929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493333
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC114442.3197312971157413No Hit
GTACATGGGAAGCAGTGGTATCAAC110092.231555561861866No Hit
GTATCAACGCAGAGTACTTTTTTTT74301.5060820987041208No Hit
GGTATCAACGCAGAGTACTTTTTTT56991.1552034832455969No Hit
GAGTACATGGGAAGCAGTGGTATCA50601.0256763687002493No Hit
TATCAACGCAGAGTACTTTTTTTTT47170.9561492946954693No Hit
CATGTACTCTGCGTTGATACCACTG42420.859865445855031No Hit
GCGTTGATACCACTGCTTCCCATGT36400.7378383363772543No Hit
ACGCAGAGTACATGGGAAGCAGTGG36300.7358113079806136No Hit
TATCAACGCAGAGTACATGGGAAGC31180.6320274540726041No Hit
GTATCAACGCAGAGTACATGGGAAG30810.6245274490050331No Hit
GGTATCAACGCAGAGTACATGGGAA29450.5969598628107181No Hit
ACTCTGCGTTGATACCACTGCTTCC28800.5837841782325529No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27600.5594598374728632No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23600.4783787016072308No Hit
GCTTCCCATGTACTCTGCGTTGATA21080.42729758601188245No Hit
GTGGTATCAACGCAGAGTACATGGG19720.3997299998175674No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18290.3707434937456039No Hit
GTACTCTGCGTTGATACCACTGCTT16940.34337861039095297No Hit
ATACCACTGCTTCCCATGTACTCTG16460.3336488740870771No Hit
GGGAAGCAGTGGTATCAACGCAGAG15150.30709480209108253No Hit
CATGGGAAGCAGTGGTATCAACGCA14420.2922974947956046No Hit
GATACCACTGCTTCCCATGTACTCT14400.29189208911627645No Hit
CAGTGGTATCAACGCAGAGTACATG14000.28378397552971324No Hit
CTGCTTCCCATGTACTCTGCGTTGA13740.27851370169844714No Hit
GCAGAGTACATGGGAAGCAGTGGTA13650.2766893761414704No Hit
GCAGTGGTATCAACGCAGAGTACAT12880.2610812574873361No Hit
TACCACTGCTTCCCATGTACTCTGC12470.2527704410611088No Hit
ACATGGGAAGCAGTGGTATCAACGC12270.2487163842678272No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11590.23493259117066972No Hit
CTTCCCATGTACTCTGCGTTGATAC10930.22155420375284035No Hit
CCACTGCTTCCCATGTACTCTGCGT10830.21952717535619956No Hit
GTTGATACCACTGCTTCCCATGTAC10590.2146623072042616No Hit
CCATGTACTCTGCGTTGATACCACT10380.21040554757131594No Hit
GTGGTATCAACGCAGAGTACTTTTT10350.20979743905232368No Hit
ATGGGAAGCAGTGGTATCAACGCAG9180.18608120681162624No Hit
AAGCAGTGGTATCAACGCAGAGTAC9010.18263525853733686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8800.17837849890439114No Hit
ATCAACGCAGAGTACTTTTTTTTTT8460.1714866023558124No Hit
TTCCCATGTACTCTGCGTTGATACC7780.1577028092586549No Hit
ATCAACGCAGAGTACATGGGAAGCA7560.15324334678604512No Hit
TGATACCACTGCTTCCCATGTACTC7440.15081091271007616No Hit
GTACATGGTAAGCAGTGGTATCAAC6140.12445954355374564No Hit
GGAAGCAGTGGTATCAACGCAGAGT5140.10418925958733756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCC250.00602934218.99837911
TATACCA550.003062976512.0911025
CTCCTAG802.8329798E-511.8848291
CAACACT600.005865331311.083514
AGCTTCT600.005869759211.08238715
TGGCCTC803.7602917E-410.6876725
GGAGGAT750.00264174510.1334966
GTACTGG856.5261195E-410.0671481
AACAGAT856.5811287E-410.0579647
GTACATA951.6250348E-410.0082761
ATAGCTG800.00450940529.4991898
GACTGTG800.00450940529.4991897
TGCACTG850.007418818.9413195
CGCCTCC850.00742498278.94041311
GAACAAA1606.61159E-78.9136221
CTGGCCT1201.756448E-48.7084724
GTGCTGG1000.00287556748.55707551
CTGGGCA1252.731903E-48.3601334
AGTACTC1605.7574987E-57.7188745
TGCGTTG33400.07.4514612
Produced by FastQC (version 0.11.5)