FastQCFastQC Report
Wed 25 May 2016
SRR1294928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4335207
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC903.652349E-813.7199968
CCGTTAG751.4594436E-512.6839341
GTACCGT752.0785756E-411.3981516
TAGACCG700.001492341710.8571375
TACCGTG1659.331416E-1010.3619577
CCGTCCA1303.8553299E-710.229119
CCGAAAC750.00265099510.13250817
TATACCG1054.1088417E-59.9523745
TAGGCCG900.00111436629.4999945
ATACCGT1201.7053375E-59.498466
GTATCAA46800.09.33000851
TAGGACT5500.09.1553864
CCTATAC4600.09.0876863
TGTACCG1151.1115153E-49.0869515
TGTCCGC850.0074477278.93993310
GTCCTAG5400.08.8082871
GTCTTGG7500.08.6250751
CGTGCAC1451.6936629E-58.51605710
GTATTAA8300.08.4814261
CTAAGAC4950.08.4451223
Produced by FastQC (version 0.11.5)