FastQCFastQC Report
Wed 25 May 2016
SRR1294928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4335207
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT124250.28660684484039634No Hit
GGTATCAACGCAGAGTACTTTTTTT71900.16585136534426154No Hit
TATCAACGCAGAGTACTTTTTTTTT67530.15577110850762144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG550.00307118412.0902719
GTATCAA56850.09.3461451
GGTATCA31150.09.3371541
GTATAGG4300.09.0628881
GTACCGT950.00182494018.999946
TACGCTG950.00182494018.999945
CTAATAC5450.08.8903613
CGTATAC1000.0029052318.5504363
CTAAGAC4000.08.5504363
ACCGTCC1000.0029079628.5495488
GTATTAG6450.08.5471131
CCGTGCA1451.6918268E-58.5167919
CTTACAC5750.08.4265173
TTACACT7800.08.403794
GTCTTAG4000.08.3168561
GTCTTAT5900.08.2161691
GTATTAA8450.08.2114031
TCAGGAC6250.08.2084193
GTCTAAT4750.08.2042981
GACTGTG7900.08.1767847
Produced by FastQC (version 0.11.5)