FastQCFastQC Report
Wed 25 May 2016
SRR1294927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300501
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC168545.6086335819181965No Hit
CCCATGTACTCTGCGTTGATACCAC159995.32410873840686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA124024.127107730090748No Hit
GAGTACATGGGAAGCAGTGGTATCA80692.685182412038562No Hit
CATGTACTCTGCGTTGATACCACTG63572.1154671698263896No Hit
GCGTTGATACCACTGCTTCCCATGT56341.874868968822067No Hit
ACGCAGAGTACATGGGAAGCAGTGG52741.7550690347120308No Hit
GGTATCAACGCAGAGTACATGGGAA50361.675867967161507No Hit
TATCAACGCAGAGTACATGGGAAGC49151.635601878196745No Hit
GTATCAACGCAGAGTACATGGGAAG47691.58701634936323No Hit
ACTCTGCGTTGATACCACTGCTTCC43021.4316092126149331No Hit
GCTTCCCATGTACTCTGCGTTGATA32601.084854958885328No Hit
GTGGTATCAACGCAGAGTACATGGG32111.0685488567425734No Hit
GTACTCTGCGTTGATACCACTGCTT29790.99134445476055No Hit
GATACCACTGCTTCCCATGTACTCT25080.8346062076332526No Hit
GGGAAGCAGTGGTATCAACGCAGAG23720.7893484547472388No Hit
ATACCACTGCTTCCCATGTACTCTG23640.786686233989238No Hit
CAGTGGTATCAACGCAGAGTACATG20750.6905135091064589No Hit
CTGCTTCCCATGTACTCTGCGTTGA20720.6895151763222086No Hit
CATGGGAAGCAGTGGTATCAACGCA20610.6858546227799575No Hit
GCAGAGTACATGGGAAGCAGTGGTA20230.6732090741794536No Hit
TACCACTGCTTCCCATGTACTCTGC20070.667884632663452No Hit
GCAGTGGTATCAACGCAGAGTACAT19430.6465868665994456No Hit
GTTGATACCACTGCTTCCCATGTAC18740.6236252125616886No Hit
GTATCAACGCAGAGTACTTTTTTTT18640.6202974366141877No Hit
CTTCCCATGTACTCTGCGTTGATAC18030.5999980033344315No Hit
ACATGGGAAGCAGTGGTATCAACGC17610.5860213443549273No Hit
CCACTGCTTCCCATGTACTCTGCGT16700.5557385832326681No Hit
CCATGTACTCTGCGTTGATACCACT15980.5317785964106609No Hit
GGTATCAACGCAGAGTACTTTTTTT14930.4968369489619003No Hit
AAGCAGTGGTATCAACGCAGAGTAC14670.4881847314983977No Hit
ATGGGAAGCAGTGGTATCAACGCAG14070.4682180758133916No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12340.4106475519216242No Hit
TGATACCACTGCTTCCCATGTACTC12100.4026608896476218No Hit
TATCAACGCAGAGTACTTTTTTTTT11940.3973364481316202No Hit
GTACATGGTAAGCAGTGGTATCAAC11870.3950070049683695No Hit
TTCCCATGTACTCTGCGTTGATACC11800.3926775618051188No Hit
ATCAACGCAGAGTACATGGGAAGCA11420.38003201320461494No Hit
ACCATGTACTCTGCGTTGATACCAC8810.2931770609748387No Hit
GGAAGCAGTGGTATCAACGCAGAGT8110.26988262934233165No Hit
AAAAAGTACTCTGCGTTGATACCAC6990.23261153873032037No Hit
CTGCGTTGATACCACTGCTTCCCAT6340.21098099507156384No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6220.20698766393456258No Hit
TGGGAAGCAGTGGTATCAACGCAGA5960.19833544647106No Hit
TCAACGCAGAGTACATGGGAAGCAG5860.195007670523559No Hit
TCCATGTACTCTGCGTTGATACCAC5810.1933437825498085No Hit
CTCTGCGTTGATACCACTGCTTCCC5760.19167989457605797No Hit
AAACAAAAAAAAAAAAAAAAAAAAA5550.18469156508630588No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5390.17936712357030427No Hit
CCCCATGTACTCTGCGTTGATACCA5290.17603934762280327No Hit
GGTAAGCAGTGGTATCAACGCAGAG4980.16572324218555012No Hit
AAAGTACTCTGCGTTGATACCACTG4960.16505768699604992No Hit
AGTGGTATCAACGCAGAGTACATGG4940.16439213180654974No Hit
TCCCATGTACTCTGCGTTGATACCA4570.152079360800796No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA4520.15041547282704548No Hit
GAGTACATGGTAAGCAGTGGTATCA4480.1490843624480451No Hit
GTACATGGAAAGCAGTGGTATCAAC4410.1467549192847944No Hit
ACTGCTTCCCATGTACTCTGCGTTG4050.13477492587379078No Hit
ATGTACTCTGCGTTGATACCACTGC3980.13244548271054007No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3980.13244548271054007No Hit
GCTTACCATGTACTCTGCGTTGATA3960.13177992752103987No Hit
AACGCAGAGTACATGGGAAGCAGTG3950.13144714992628978No Hit
GTACATGGGTAAGCAGTGGTATCAA3810.12678826359978837No Hit
TTGATACCACTGCTTCCCATGTACT3660.12179659967853683No Hit
ACTCTGCGTTGATACCACTGCTTAC3600.11979993411003623No Hit
GAACAAAAAAAAAAAAAAAAAAAAA3410.11347715980978433No Hit
CAACGCAGAGTACATGGGAAGCAGT3320.11048216145703342No Hit
GCGTTGATACCACTGCTTACCATGT3270.10881827348328292No Hit
GTGGTATCAACGCAGAGTACTTTTT3160.1051577199410318No Hit
GAGTACATGGAAAGCAGTGGTATCA3110.10349383196728129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGT250.006017027419.00216716
CCATAGG250.00602282218.99915
ACATAAA402.7494223E-416.6268943
GTAAAAA601.4403686E-615.8509851
GTACAAA801.2593591E-714.2658851
GAACAAA2350.013.7600021
GTCAGTC550.003052114312.09430218
CGCAAAA550.003055927612.0922872
GGTCAGT550.00305974512.09027317
TCGGTCA657.993907E-411.69169215
TACAAAA1251.803528E-811.4012992
ACGCAAA600.00580856111.0956891
CATAAAA600.0058519911.0845974
AAAAGTA1752.0008883E-1110.8583812
AGAACAA700.001483924810.858382
GAGAACA909.3493174E-510.5673231
GAGAAAA951.6155327E-410.01114751
AGTACTC2002.5465852E-119.9744765
GTACTGG1054.024685E-59.9634761
ATCGGTC1151.0179629E-59.91252214
Produced by FastQC (version 0.11.5)