##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294927_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	300501
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.403672533535662	31.0	30.0	34.0	16.0	34.0
2	29.48799504826939	31.0	30.0	34.0	16.0	34.0
3	29.942945281380094	31.0	30.0	34.0	23.0	34.0
4	32.92044951597499	37.0	35.0	37.0	19.0	37.0
5	33.88081570444025	37.0	35.0	37.0	28.0	37.0
6	33.58485995054925	37.0	35.0	37.0	26.0	37.0
7	33.820459832080424	37.0	35.0	37.0	27.0	37.0
8	33.82416364670999	37.0	35.0	37.0	27.0	37.0
9	34.97468228059141	39.0	35.0	39.0	25.0	39.0
10	35.229147323968974	39.0	35.0	39.0	27.0	39.0
11	35.21315403276528	39.0	35.0	39.0	27.0	39.0
12	35.39440800529782	39.0	35.0	39.0	27.0	39.0
13	34.93766742872736	39.0	35.0	39.0	25.0	39.0
14	36.11287483236329	40.0	36.0	41.0	25.0	41.0
15	36.05140415506105	40.0	36.0	41.0	25.0	41.0
16	35.890076904902145	40.0	36.0	41.0	25.0	41.0
17	36.00972708909455	40.0	36.0	41.0	25.0	41.0
18	35.83304880848982	40.0	36.0	41.0	25.0	41.0
19	35.74355160215773	40.0	35.0	41.0	24.0	41.0
20	35.800842592869905	40.0	35.0	41.0	24.0	41.0
21	35.68796443273067	40.0	35.0	41.0	24.0	41.0
22	35.80759132249144	40.0	35.0	41.0	24.0	41.0
23	35.789997371057	40.0	35.0	41.0	24.0	41.0
24	35.94005344408172	40.0	35.0	41.0	25.0	41.0
25	34.90388051953238	39.0	33.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40532650010692706
1101	2	0.17130999230306898
1101	3	0.014430824368044881
1101	4	0.26556205261874055
1101	5	-0.07928732399332716
1101	6	-0.17865519614220204
1101	7	-0.0832643774620152
1101	8	0.3642555943546526
1101	9	0.3730487978952297
1101	10	0.30266521324684703
1101	11	0.2468342550438365
1101	12	0.33776476187365034
1101	13	0.6101053201645357
1101	14	0.33755203013429735
1101	15	0.3285528509695581
1101	16	0.4501734123872865
1101	17	-0.020020747069224853
1101	18	-0.07402452440556573
1101	19	0.21072173116557025
1101	20	-0.15362345795508503
1101	21	-0.3471627229684131
1101	22	-0.531212164405126
1101	23	-0.06005418410435226
1101	24	-0.1610276356116458
1101	25	0.0012201651634242694
1102	1	-0.05149661575509157
1102	2	0.04754817060074501
1102	3	0.042856250962810094
1102	4	0.023708559048998268
1102	5	0.06101021218521652
1102	6	0.2219411563992324
1102	7	-0.07546592115097184
1102	8	0.16876611053564972
1102	9	0.3903366206750434
1102	10	0.2814514918042974
1102	11	0.29371567263464016
1102	12	0.4764834040247834
1102	13	0.25412339210023305
1102	14	0.888057969656252
1102	15	0.703593523493069
1102	16	0.8195279327715568
1102	17	0.07944424255594384
1102	18	0.012019865986651723
1102	19	-0.08507553030675297
1102	20	-0.05865511584715932
1102	21	-0.043417650344622416
1102	22	0.14395705996318497
1102	23	-0.06641243302553335
1102	24	-0.07367639036566231
1102	25	0.5359691690199782
1103	1	-0.20499997514080093
1103	2	-0.09807091619481412
1103	3	-0.22424919261322174
1103	4	0.06018188963997062
1103	5	-0.1083988101649851
1103	6	-0.20079297221725056
1103	7	-0.06141134827286976
1103	8	6.597071351279737E-4
1103	9	-0.10774373034648477
1103	10	-0.03769015086675864
1103	11	0.0026146168276994786
1103	12	0.056217395523482594
1103	13	-0.16518279191183893
1103	14	-0.042491872658295904
1103	15	-0.22304846197244643
1103	16	0.11865037895282882
1103	17	-0.015029910741738206
1103	18	0.25924323085707357
1103	19	0.21199014911186964
1103	20	0.06591639107729463
1103	21	-0.024632513912422382
1103	22	-0.09915277138193801
1103	23	-0.024522949959575158
1103	24	-0.21627888590933253
1103	25	0.16184329489410487
1104	1	-0.034608741724358794
1104	2	-0.08822082231110429
1104	3	-0.13658335704822733
1104	4	-0.08505569257239642
1104	5	0.0883003823325268
1104	6	0.14236993397086906
1104	7	-0.5178965064387029
1104	8	-0.036124237211836885
1104	9	-0.10361387327545657
1104	10	0.302779370275033
1104	11	0.10951891700931071
1104	12	0.12063290531714443
1104	13	0.14039584980125852
1104	14	-0.0016396343270628222
1104	15	0.09624848757660232
1104	16	-0.04467135999916394
1104	17	-0.10581494270324043
1104	18	0.06666363491613225
1104	19	-0.2599165622906412
1104	20	-0.08190171052232387
1104	21	-0.21599110863236604
1104	22	-0.3630121612917492
1104	23	-0.26784592017330766
1104	24	-0.017573762834466322
1104	25	-0.2940262324039935
1105	1	-0.017962805697578688
1105	2	-0.3178088200384117
1105	3	-0.18962308309665943
1105	4	-0.24896083325922547
1105	5	-0.525428931816009
1105	6	0.03321415728848365
1105	7	-0.09682905763826
1105	8	0.04630692938572878
1105	9	0.127422709905602
1105	10	-0.38339457259030496
1105	11	-0.397260770034805
1105	12	-0.19978088849232734
1105	13	-0.3497430198925926
1105	14	-0.2840626110422022
1105	15	-0.549933235677031
1105	16	-0.33727538430264303
1105	17	-0.1661897321976724
1105	18	-0.2939676002716993
1105	19	-0.12861739100436864
1105	20	-0.27000283509555345
1105	21	-0.12759156521831017
1105	22	-0.24506249835659588
1105	23	-0.2811698351418528
1105	24	-0.030339742062871267
1105	25	-0.007370110210949576
1106	1	-0.07587114832634256
1106	2	-0.5122199301509625
1106	3	0.01909858786088492
1106	4	-0.38418955376715047
1106	5	-0.5553188139798806
1106	6	-0.4255643435652843
1106	7	-0.49852919667326034
1106	8	-0.34670748690516007
1106	9	-0.3482144457448797
1106	10	-0.269058107885634
1106	11	-0.18902017075694033
1106	12	-0.5500856553311593
1106	13	-0.6537010239209096
1106	14	-0.8686334263794038
1106	15	-0.5318322529163169
1106	16	-0.5590388556313002
1106	17	-0.601781635391248
1106	18	-0.3869254509914555
1106	19	-0.1572437991963298
1106	20	0.052294044282334085
1106	21	-0.2069208138477805
1106	22	-0.39055760569902276
1106	23	0.037347195324258564
1106	24	-0.23633886450354424
1106	25	-0.03388720411508217
1107	1	0.1037372746482923
1107	2	0.189393613293646
1107	3	0.08969359441032054
1107	4	0.0802946283469268
1107	5	0.4099691628390829
1107	6	0.30758573780652654
1107	7	0.02396713167190967
1107	8	0.19483679126734899
1107	9	0.25531133522602545
1107	10	0.6203357713665412
1107	11	0.31756399944906377
1107	12	0.18711316973929826
1107	13	0.3288735007486423
1107	14	0.28489136225235967
1107	15	0.5538611936759921
1107	16	-0.10646274321911875
1107	17	0.09320308258597265
1107	18	0.5131162272525032
1107	19	0.11098547083899746
1107	20	0.06302123040546093
1107	21	0.1060331813096056
1107	22	-0.057077075392982124
1107	23	0.21311030412432075
1107	24	-0.12066189074099043
1107	25	0.10072856216920201
1108	1	-0.2065082707667436
1108	2	-0.22401360096102607
1108	3	0.25614296424952343
1108	4	-0.3067110213705817
1108	5	-0.006588855413852457
1108	6	0.0733397577978323
1108	7	-0.02923437491942593
1108	8	0.1454560872256252
1108	9	0.0432115235499424
1108	10	0.23619119654399867
1108	11	0.34499772391669126
1108	12	0.18530236284374269
1108	13	0.1536960416728803
1108	14	0.1232949548932396
1108	15	-0.05864423874469793
1108	16	-0.15774479450116985
1108	17	-0.025839362727658965
1108	18	-0.09093273696595361
1108	19	-0.11304755827877955
1108	20	-0.2249332821253205
1108	21	-0.02865463558160286
1108	22	-0.13032421431808672
1108	23	-0.15071701733011622
1108	24	0.15262508593581714
1108	25	0.217398850449662
1109	1	0.05122641878612555
1109	2	0.08076495381752835
1109	3	-0.09356036272653157
1109	4	0.16987300066798383
1109	5	0.4055217612422197
1109	6	0.20403482031107956
1109	7	0.3745764607095907
1109	8	0.4248585029916825
1109	9	0.17016285504672624
1109	10	0.3462427611410206
1109	11	-0.09052887070081539
1109	12	-0.15474143174550647
1109	13	-0.024537341105954624
1109	14	-0.14097360482986687
1109	15	0.1322860430946804
1109	16	0.1394050253759218
1109	17	0.12034273799989137
1109	18	0.18271280940686552
1109	19	-0.08830714717473143
1109	20	-0.07481320018705873
1109	21	0.0525242865064115
1109	22	0.3826728895881075
1109	23	0.31396476553348407
1109	24	0.32624944137835143
1109	25	0.11972632095895364
1110	1	0.14939860354503764
1110	2	-0.0063794968238539695
1110	3	-0.021896991704544178
1110	4	-0.06182349580821267
1110	5	-0.049061719972606
1110	6	-0.012117218763435744
1110	7	0.0460894151282929
1110	8	0.08740983899533461
1110	9	0.05462938312731325
1110	10	0.49214264114574746
1110	11	-0.0337973051850895
1110	12	0.37494894666210854
1110	13	-0.062378102315378214
1110	14	0.11754014392370493
1110	15	0.036526439248071085
1110	16	-0.007791986572904364
1110	17	0.01298922518068224
1110	18	0.18684504081182496
1110	19	0.2799050923425881
1110	20	0.20461944190592618
1110	21	0.05775929132312285
1110	22	0.017439405838167943
1110	23	0.2706819654185608
1110	24	0.3142710405265561
1110	25	0.15327006852946568
1111	1	-0.4529058126188339
1111	2	-0.3138631453560272
1111	3	-0.19686614785049983
1111	4	0.08516225686633305
1111	5	-0.015965842063565105
1111	6	-0.2856346011765254
1111	7	0.17209712186661363
1111	8	0.09427999059498404
1111	9	0.1660306236417739
1111	10	-0.06698037935485246
1111	11	-0.07399994508097762
1111	12	-0.14854308463806376
1111	13	-0.373710894824967
1111	14	-0.04593228251581394
1111	15	-0.42143296516978523
1111	16	-0.058942082062095835
1111	17	-0.005690319851346715
1111	18	0.20130785072917945
1111	19	0.46954409249469364
1111	20	0.5181620064245109
1111	21	0.20128461708492296
1111	22	0.03763156727405459
1111	23	0.08669203826075744
1111	24	0.17748911079983998
1111	25	0.4151808664134933
1112	1	-0.349506298380156
1112	2	-0.10271182581309901
1112	3	-0.24385703575047657
1112	4	-0.34566565647906344
1112	5	-0.36950798756375747
1112	6	-0.2950034374684378
1112	7	-0.13212720858148685
1112	8	-0.24113168397752816
1112	9	-0.5739811128734615
1112	10	-0.36029138070780675
1112	11	-0.48340464294721386
1112	12	-0.1261703524751212
1112	13	-0.5328588564204821
1112	14	-0.5376189175590937
1112	15	-0.5596304320385812
1112	16	-0.8130702382881552
1112	17	-0.48573416877631104
1112	18	-0.7401766554406422
1112	19	-0.4818838501919913
1112	20	-0.5076420263725296
1112	21	-0.5664587118472184
1112	22	-0.4669448586310381
1112	23	-0.6323080758442501
1112	24	-0.24366025265721447
1112	25	-0.49003047236427477
1113	1	-0.3674542305174846
1113	2	-0.3742332352444073
1113	3	-0.47452411103906655
1113	4	-0.7008013268154727
1113	5	-0.25911720355973245
1113	6	-0.5754752386267228
1113	7	-0.5044446449504107
1113	8	-0.7397722302736085
1113	9	-0.7656640851264598
1113	10	-0.716621484356331
1113	11	-0.8632973011114018
1113	12	-0.9107833796563369
1113	13	-0.8576347037216081
1113	14	-0.938807064380093
1113	15	-0.7162701453745868
1113	16	-0.8026778826613494
1113	17	-0.8931734452011852
1113	18	-0.8968202978335924
1113	19	-0.7010545048514629
1113	20	-0.7682131540904535
1113	21	-0.6374842742917224
1113	22	-0.7132574386855737
1113	23	-0.7728779462639537
1113	24	-0.7336843770668722
1113	25	-0.7737987523017225
1114	1	0.09324278723673984
1114	2	0.26289205002229465
1114	3	0.33647654652485315
1114	4	0.48665467442229726
1114	5	0.184150502315795
1114	6	0.2721323891147378
1114	7	0.18058223780980853
1114	8	0.05344712531375251
1114	9	0.21079512657043864
1114	10	-0.15756450837675118
1114	11	-0.1111138020977478
1114	12	0.20625912991701512
1114	13	0.02803427086547572
1114	14	0.07370985822076648
1114	15	0.2621424179642915
1114	16	0.2697508490138887
1114	17	0.12019831049448015
1114	18	-0.20927146404254415
1114	19	0.3092576446089481
1114	20	0.3915632741770594
1114	21	0.21499319447399046
1114	22	0.2764304118540153
1114	23	0.4148874968180891
1114	24	0.2285482458395549
1114	25	-0.015229147226882844
1115	1	0.1553787095668362
1115	2	0.3172609820611285
1115	3	-0.007212773863496835
1115	4	0.11966438316016337
1115	5	-0.2333252258395433
1115	6	-0.04825596039982827
1115	7	-0.2621362087921355
1115	8	-0.3815043309969255
1115	9	-0.20862234915771438
1115	10	-0.39834120740587764
1115	11	-0.17130797685503296
1115	12	-0.2694690254227865
1115	13	-0.12730553495976693
1115	14	-0.3029891709054482
1115	15	-0.32232360145318495
1115	16	-0.5962685684618378
1115	17	-0.2895104273695992
1115	18	-0.3296598135571074
1115	19	-0.5587035204395931
1115	20	-0.3191163374734245
1115	21	-0.27626894144833614
1115	22	0.0453624506889625
1115	23	-0.03753968764792859
1115	24	-0.4102867056167554
1115	25	-0.356976720042411
1116	1	-0.17632768611145266
1116	2	-0.2707560932288118
1116	3	-0.19616672346872477
1116	4	-0.4153361834976934
1116	5	-0.3002135186883237
1116	6	-0.5511988035111557
1116	7	-0.5860869473361987
1116	8	-0.6725624700925366
1116	9	-1.135236379606134
1116	10	-0.9182120094156261
1116	11	-0.758913280772525
1116	12	-0.7342909059713136
1116	13	-0.6238073671534892
1116	14	-0.7742822841236858
1116	15	-0.9337814232204025
1116	16	-0.9128829766821553
1116	17	-0.7200759519058835
1116	18	-1.1010927023788355
1116	19	-0.8503018733541055
1116	20	-0.9408533326757222
1116	21	-0.9623404914464473
1116	22	-0.6009750507276763
1116	23	-0.7696767432937364
1116	24	-1.0592459440286035
1116	25	-1.1012515868778152
1201	1	-0.37270553650634497
1201	2	-0.4122140351568859
1201	3	-0.06337237929328055
1201	4	-0.4323784508137578
1201	5	-0.1273422439017935
1201	6	-0.37316320529551916
1201	7	-0.06038108226750438
1201	8	0.15601760808463183
1201	9	-0.13038369638395153
1201	10	-0.3083187684465116
1201	11	-0.40076299896073664
1201	12	-0.22974095895014557
1201	13	-0.165638386929281
1201	14	0.33297537109539377
1201	15	0.1912530798025074
1201	16	0.36550522016760567
1201	17	0.0211691842808861
1201	18	0.042680281086425964
1201	19	-0.15769932146601207
1201	20	0.04774953975658747
1201	21	-0.024508260725845332
1201	22	-0.014049693009241082
1201	23	0.026902337634773232
1201	24	-0.4402038369846437
1201	25	0.04698675555243881
1202	1	0.042985275566529424
1202	2	-0.27717676586233964
1202	3	0.022566533156162194
1202	4	0.015147518885250122
1202	5	-0.186828480348737
1202	6	-0.3437610120117398
1202	7	-0.25032792897008704
1202	8	-0.08913325120118287
1202	9	0.09807733744510472
1202	10	-0.15991827462190855
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2209	23	0.1304831854802586
2209	24	0.15688048214036598
2209	25	-0.058169742915538336
2210	1	-0.1537839117923845
2210	2	0.1580376810902564
2210	3	0.19428398989054685
2210	4	0.23703897862941403
2210	5	0.3285072984322994
2210	6	-0.15855126784319395
2210	7	0.11729340285835121
2210	8	-0.0588708358512946
2210	9	0.006405967994389528
2210	10	0.3506676922704983
2210	11	0.18073490340386655
2210	12	0.3218942431856249
2210	13	0.19338621261304922
2210	14	0.2625043566026406
2210	15	0.0982467868963326
2210	16	0.28937059011421695
2210	17	0.3711691842808875
2210	18	0.2889283741451578
2210	19	0.05201654428532265
2210	20	0.23703252984049072
2210	21	0.3270077575807875
2210	22	0.3815881788443036
2210	23	0.2309069142938185
2210	24	0.3568451714059009
2210	25	0.016971500022314956
2211	1	0.12964451144412692
2211	2	-0.01872580438692495
2211	3	0.046652592933423165
2211	4	0.047714981395664324
2211	5	-0.3631161455511034
2211	6	0.2776434071222269
2211	7	0.036925145596939046
2211	8	0.01698497327733861
2211	9	0.1290805876347747
2211	10	-0.17022615281941
2211	11	-0.13180831927939352
2211	12	0.17387384207912504
2211	13	0.09953247817321653
2211	14	0.07860048385022367
2211	15	0.060357782746955024
2211	16	0.021130617776734084
2211	17	0.10415673614810572
2211	18	0.14950064247157258
2211	19	-0.023623151426981792
2211	20	0.16448757824989713
2211	21	0.5678272596554805
2211	22	0.2898091332011532
2211	23	0.2643094039203717
2211	24	0.30206474263687966
2211	25	0.46586499933074776
2212	1	0.22273085399172743
2212	2	0.3747382920882174
2212	3	0.38802974684913494
2212	4	0.3197316127095249
2212	5	0.25927359171132025
2212	6	0.19959961470942034
2212	7	0.39518291405997985
2212	8	0.1062895511140809
2212	9	0.42740477994279047
2212	10	0.09631008874028169
2212	11	-0.04849540888397996
2212	12	0.19072910401386878
2212	13	0.18665250589207005
2212	14	0.5669256057540295
2212	15	0.423208599523619
2212	16	0.3858997822391288
2212	17	0.25131175047233256
2212	18	0.541223859549099
2212	19	0.4226462116308767
2212	20	0.24147923724035536
2212	21	0.13436519138934244
2212	22	0.4542842073575457
2212	23	0.680092250342696
2212	24	0.6557801680114679
2212	25	0.44063069893608997
2213	1	0.3900406408915096
2213	2	0.5441211800962193
2213	3	0.5346004246155296
2213	4	0.42208288187659804
2213	5	0.6005376165237521
2213	6	0.822218281527249
2213	7	0.31017362154622674
2213	8	0.43510282220039187
2213	9	0.3314266770732459
2213	10	0.4832430733175457
2213	11	0.5973808609916773
2213	12	0.5018602802962917
2213	13	0.4583340257343238
2213	14	0.5400805660128469
2213	15	0.2174938047889725
2213	16	0.45765256493197626
2213	17	0.4903540749369526
2213	18	0.10931024955934276
2213	19	0.3785158815948009
2213	20	0.33521013090079776
2213	21	0.3645077575807889
2213	22	0.5352039840132932
2213	23	0.6824973715502765
2213	24	0.8863721760447305
2213	25	0.44284956496598227
2214	1	0.7125863286885767
2214	2	0.5540296650581915
2214	3	0.6842555997769857
2214	4	1.1199756853762395
2214	5	0.7544717272900172
2214	6	0.7108583847954151
2214	7	0.7856175511549424
2214	8	0.8536416891988026
2214	9	0.8485444115481613
2214	10	0.6368943121769775
2214	11	0.6614254511660604
2214	12	0.6950697608876979
2214	13	0.7620109621120505
2214	14	0.9136149110448599
2214	15	0.5441510955135627
2214	16	0.9671845513700603
2214	17	0.8989246953029308
2214	18	0.9183079666635265
2214	19	1.2487015810254718
2214	20	0.9510939860863132
2214	21	1.1360508437531323
2214	22	1.1646417860587306
2214	23	0.8700852710072411
2214	24	0.7479482108180591
2214	25	0.49890202774442116
2215	1	0.40488388317860213
2215	2	0.3259293638755558
2215	3	0.12092003115424887
2215	4	0.5316190882361198
2215	5	0.40257644736847453
2215	6	-0.23389378879741685
2215	7	-0.01838116193854944
2215	8	0.20303680438081528
2215	9	0.011027986047260185
2215	10	-0.1557354347443365
2215	11	-0.3646189005935554
2215	12	-0.19048083740754151
2215	13	0.10364975226488582
2215	14	0.13316038497143268
2215	15	-0.11424112412881726
2215	16	0.273131067225755
2215	17	0.2339351417276916
2215	18	-0.0027100849135166527
2215	19	0.1545713476379973
2215	20	0.11443420617833766
2215	21	0.0530957653177353
2215	22	0.16032367207447606
2215	23	-0.015031190154928709
2215	24	0.30950071621570174
2215	25	0.457558218268602
2216	1	0.44297992219970794
2216	2	0.395982345121876
2216	3	0.0558287856744748
2216	4	0.3392046571537932
2216	5	0.1907355593018636
2216	6	0.4801756755033182
2216	7	0.05289122894530607
2216	8	0.23818944082854898
2216	9	0.22316321437119768
2216	10	0.06821545248129723
2216	11	0.06335183357514751
2216	12	0.2043135448984046
2216	13	-0.2178539059640272
2216	14	-0.08204439175045763
2216	15	-0.1318174423526841
2216	16	-0.2220753914404625
2216	17	-0.10728931374283235
2216	18	0.18684504081182496
2216	19	0.16869967999019764
2216	20	0.22998378148739107
2216	21	0.40205716469541386
2216	22	0.369557546433235
2216	23	0.5872310249657531
2216	24	0.21602666020695693
2216	25	-0.1455449690158943
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89.0
3	51.0
4	9.0
5	13.0
6	11.0
7	34.0
8	32.0
9	78.0
10	182.0
11	311.0
12	687.0
13	1123.0
14	1962.0
15	3077.0
16	3874.0
17	4304.0
18	4294.0
19	3837.0
20	3582.0
21	3183.0
22	2927.0
23	2905.0
24	2634.0
25	2506.0
26	2421.0
27	2445.0
28	2428.0
29	2621.0
30	3233.0
31	4072.0
32	5280.0
33	7049.0
34	10033.0
35	14829.0
36	25482.0
37	52160.0
38	126743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33426959740645	23.52988215067521	10.948292234339235	21.187556017579105
2	11.88604846337272	30.00486232299146	31.02461800791293	27.08447120572289
3	21.05720535794552	37.58186582495114	24.196990733804135	17.163938083299204
4	12.952261683114566	29.828038142692037	24.72380520981478	32.495894964378614
5	9.568735846543227	46.68409484481151	30.48687891301452	13.260290395630744
6	28.375673902691616	35.86411058164587	16.885945195352694	18.874270320309822
7	18.714328527188574	42.73850354633545	20.075255569245112	18.47191235723086
8	25.810404886073755	39.623420651254484	19.061748622961083	15.504425839710672
9	25.30545777872502	25.98647294088655	17.130944729542072	31.577124550846356
10	17.62063488363575	39.50057774211057	25.413993013815862	17.464794360437818
11	22.71897810218978	35.817704725877775	15.00912408759124	26.4541930843412
12	22.904477264023708	41.06228874939642	22.707337784511896	13.325896202067966
13	28.017249991674714	29.3716074461354	18.909054580572114	23.70208798161777
14	19.981086462835567	37.75448364045632	21.240435011354784	21.023994885353332
15	33.37706446457113	28.167288225892385	19.0200452850293	19.435602024507194
16	14.741602368353574	35.03145261461306	31.06366115760274	19.16328385943062
17	24.42395239094309	37.243696695406605	22.53670752866501	15.795643384985297
18	24.318059963434493	29.396935557457464	26.734114152316312	19.550890326791727
19	21.497266012640946	38.25217953072656	27.332214429858738	12.91834002677376
20	23.337152144084616	30.932367085403705	24.884769605818803	20.845711164692872
21	18.650423615921564	34.56133105983908	28.247722065327434	18.54052325891192
22	22.273841399246027	33.40959650454915	18.06804406612583	26.248518030078994
23	16.632751276406545	33.70246155793204	26.132613061477333	23.53217410418408
24	20.831126787302285	40.82926163141189	20.71555373480814	17.624057846477687
25	21.135490145141844	29.365412411992352	19.459931125898393	30.039166316967407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13228.0
1	13228.0
2	9456.5
3	5685.0
4	5685.0
5	5685.0
6	5456.0
7	5227.0
8	5227.0
9	5227.0
10	4855.5
11	4484.0
12	4484.0
13	4484.0
14	4396.0
15	4308.0
16	4308.0
17	4308.0
18	4662.5
19	5017.0
20	5017.0
21	5017.0
22	5737.0
23	6457.0
24	6457.0
25	6457.0
26	7462.0
27	8467.0
28	8467.0
29	8467.0
30	8967.0
31	9467.0
32	9467.0
33	9467.0
34	9811.5
35	10156.0
36	10156.0
37	10156.0
38	10271.0
39	10386.0
40	10386.0
41	10386.0
42	14643.0
43	18900.0
44	18900.0
45	18900.0
46	56913.5
47	94927.0
48	94927.0
49	94927.0
50	78957.0
51	62987.0
52	62987.0
53	62987.0
54	42517.0
55	22047.0
56	22047.0
57	22047.0
58	14884.5
59	7722.0
60	7722.0
61	7722.0
62	6467.5
63	5213.0
64	5213.0
65	5213.0
66	4125.5
67	3038.0
68	3038.0
69	3038.0
70	2338.5
71	1639.0
72	1639.0
73	1639.0
74	1174.5
75	710.0
76	710.0
77	710.0
78	507.0
79	304.0
80	304.0
81	304.0
82	198.5
83	93.0
84	93.0
85	93.0
86	60.0
87	27.0
88	27.0
89	27.0
90	19.0
91	11.0
92	11.0
93	11.0
94	6.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12379326524703746
2	0.07753717957677346
3	0.053909970349516306
4	0.08652217463502618
5	0.07354384843977226
6	0.06588996376051993
7	0.06356052059726923
8	0.07287829325027204
9	0.07121440527652154
10	0.06455885338151952
11	0.06555718616576983
12	0.06855218451852073
13	0.07021607249227124
14	0.06222941021826882
15	0.060232744649768216
16	0.06921773970802095
17	0.07387662603452234
18	0.07254551565552195
19	0.06888496211327083
20	0.07753717957677346
21	0.07620606919777305
22	0.07487495881877265
23	0.08119773311902456
24	0.08585661944552597
25	0.08086495552427446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	300501.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.2624683445313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6014435587373	22.77995747102339
2	11.115166786374502	6.282840988881901
3	3.7007382637261714	3.137759940898699
4	1.5259805249090417	1.7251190511845218
5	0.765345170671973	1.0815271829378272
6	0.48511109279515835	0.8226262142222489
7	0.3026057059426109	0.5986668929554311
8	0.21076428546197412	0.4765375156821442
9	0.1613112128954774	0.41031477432687413
>10	0.8701385863485971	4.831597898176711
>50	0.10479341567662401	2.0638866426401243
>100	0.09772869102426733	6.47851421459496
>500	0.014129449304713349	2.532104718453516
>1k	0.03532362326178337	21.612240891045285
>5k	0.005887270543630562	10.106455552560558
>10k+	0.003532362326178337	15.059850050415804
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	16854	5.6086335819181965	No Hit
CCCATGTACTCTGCGTTGATACCAC	15999	5.32410873840686	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	12402	4.127107730090748	No Hit
GAGTACATGGGAAGCAGTGGTATCA	8069	2.685182412038562	No Hit
CATGTACTCTGCGTTGATACCACTG	6357	2.1154671698263896	No Hit
GCGTTGATACCACTGCTTCCCATGT	5634	1.874868968822067	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5274	1.7550690347120308	No Hit
GGTATCAACGCAGAGTACATGGGAA	5036	1.675867967161507	No Hit
TATCAACGCAGAGTACATGGGAAGC	4915	1.635601878196745	No Hit
GTATCAACGCAGAGTACATGGGAAG	4769	1.58701634936323	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4302	1.4316092126149331	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3260	1.084854958885328	No Hit
GTGGTATCAACGCAGAGTACATGGG	3211	1.0685488567425734	No Hit
GTACTCTGCGTTGATACCACTGCTT	2979	0.99134445476055	No Hit
GATACCACTGCTTCCCATGTACTCT	2508	0.8346062076332526	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2372	0.7893484547472388	No Hit
ATACCACTGCTTCCCATGTACTCTG	2364	0.786686233989238	No Hit
CAGTGGTATCAACGCAGAGTACATG	2075	0.6905135091064589	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2072	0.6895151763222086	No Hit
CATGGGAAGCAGTGGTATCAACGCA	2061	0.6858546227799575	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	2023	0.6732090741794536	No Hit
TACCACTGCTTCCCATGTACTCTGC	2007	0.667884632663452	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1943	0.6465868665994456	No Hit
GTTGATACCACTGCTTCCCATGTAC	1874	0.6236252125616886	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1864	0.6202974366141877	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1803	0.5999980033344315	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1761	0.5860213443549273	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1670	0.5557385832326681	No Hit
CCATGTACTCTGCGTTGATACCACT	1598	0.5317785964106609	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1493	0.4968369489619003	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1467	0.4881847314983977	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	1407	0.4682180758133916	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1234	0.4106475519216242	No Hit
TGATACCACTGCTTCCCATGTACTC	1210	0.4026608896476218	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1194	0.3973364481316202	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1187	0.3950070049683695	No Hit
TTCCCATGTACTCTGCGTTGATACC	1180	0.3926775618051188	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1142	0.38003201320461494	No Hit
ACCATGTACTCTGCGTTGATACCAC	881	0.2931770609748387	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	811	0.26988262934233165	No Hit
AAAAAGTACTCTGCGTTGATACCAC	699	0.23261153873032037	No Hit
CTGCGTTGATACCACTGCTTCCCAT	634	0.21098099507156384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	622	0.20698766393456258	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	596	0.19833544647106	No Hit
TCAACGCAGAGTACATGGGAAGCAG	586	0.195007670523559	No Hit
TCCATGTACTCTGCGTTGATACCAC	581	0.1933437825498085	No Hit
CTCTGCGTTGATACCACTGCTTCCC	576	0.19167989457605797	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	555	0.18469156508630588	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	539	0.17936712357030427	No Hit
CCCCATGTACTCTGCGTTGATACCA	529	0.17603934762280327	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	498	0.16572324218555012	No Hit
AAAGTACTCTGCGTTGATACCACTG	496	0.16505768699604992	No Hit
AGTGGTATCAACGCAGAGTACATGG	494	0.16439213180654974	No Hit
TCCCATGTACTCTGCGTTGATACCA	457	0.152079360800796	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	452	0.15041547282704548	No Hit
GAGTACATGGTAAGCAGTGGTATCA	448	0.1490843624480451	No Hit
GTACATGGAAAGCAGTGGTATCAAC	441	0.1467549192847944	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	405	0.13477492587379078	No Hit
ATGTACTCTGCGTTGATACCACTGC	398	0.13244548271054007	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	398	0.13244548271054007	No Hit
GCTTACCATGTACTCTGCGTTGATA	396	0.13177992752103987	No Hit
AACGCAGAGTACATGGGAAGCAGTG	395	0.13144714992628978	No Hit
GTACATGGGTAAGCAGTGGTATCAA	381	0.12678826359978837	No Hit
TTGATACCACTGCTTCCCATGTACT	366	0.12179659967853683	No Hit
ACTCTGCGTTGATACCACTGCTTAC	360	0.11979993411003623	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	341	0.11347715980978433	No Hit
CAACGCAGAGTACATGGGAAGCAGT	332	0.11048216145703342	No Hit
GCGTTGATACCACTGCTTACCATGT	327	0.10881827348328292	No Hit
GTGGTATCAACGCAGAGTACTTTTT	316	0.1051577199410318	No Hit
GAGTACATGGAAAGCAGTGGTATCA	311	0.10349383196728129	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.3277759475010065E-4	0.0	0.0	0.0	0.0
9	3.3277759475010065E-4	0.0	0.0	0.0	0.0
10	3.3277759475010065E-4	0.0	0.0	0.0	0.0
11	3.3277759475010065E-4	0.0	0.0	0.0	0.0
12	3.3277759475010065E-4	0.0	0.0	0.0	0.0
13	3.3277759475010065E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGGT	25	0.0060170274	19.002167	16
CCATAGG	25	0.006022822	18.999	15
ACATAAA	40	2.7494223E-4	16.626894	3
GTAAAAA	60	1.4403686E-6	15.850985	1
GTACAAA	80	1.2593591E-7	14.265885	1
GAACAAA	235	0.0	13.760002	1
GTCAGTC	55	0.0030521143	12.094302	18
CGCAAAA	55	0.0030559276	12.092287	2
GGTCAGT	55	0.003059745	12.090273	17
TCGGTCA	65	7.993907E-4	11.691692	15
TACAAAA	125	1.803528E-8	11.401299	2
ACGCAAA	60	0.005808561	11.095689	1
CATAAAA	60	0.00585199	11.084597	4
AAAAGTA	175	2.0008883E-11	10.858381	2
AGAACAA	70	0.0014839248	10.85838	2
GAGAACA	90	9.3493174E-5	10.567323	1
GAGAAAA	95	1.6155327E-4	10.0111475	1
AGTACTC	200	2.5465852E-11	9.974476	5
GTACTGG	105	4.024685E-5	9.963476	1
ATCGGTC	115	1.0179629E-5	9.912522	14
>>END_MODULE