FastQCFastQC Report
Wed 25 May 2016
SRR1294927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300501
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC178095.926436184904542No Hit
GTACATGGGAAGCAGTGGTATCAAC170475.672859657704966No Hit
GAGTACATGGGAAGCAGTGGTATCA79992.661887980406055No Hit
GTATCAACGCAGAGTACTTTTTTTT73402.442587545465739No Hit
CATGTACTCTGCGTTGATACCACTG67122.2336032159626753No Hit
GGTATCAACGCAGAGTACTTTTTTT61662.0519066492291205No Hit
GCGTTGATACCACTGCTTCCCATGT57781.9227889424660816No Hit
ACGCAGAGTACATGGGAAGCAGTGG57561.9154678353815795No Hit
TATCAACGCAGAGTACATGGGAAGC51121.7011590643625145No Hit
GGTATCAACGCAGAGTACATGGGAA49361.6425902076864969No Hit
GTATCAACGCAGAGTACATGGGAAG48931.6282807711122425No Hit
TATCAACGCAGAGTACTTTTTTTTT48331.6083141154272365No Hit
ACTCTGCGTTGATACCACTGCTTCC45691.52046083041321No Hit
GCTTCCCATGTACTCTGCGTTGATA33681.1207949391183392No Hit
GTGGTATCAACGCAGAGTACATGGG31301.041593871567815No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30511.0153044415825572No Hit
GTACTCTGCGTTGATACCACTGCTT28660.9537405865537885No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26470.8808622933035165No Hit
GATACCACTGCTTCCCATGTACTCT25550.8502467545865071No Hit
ATACCACTGCTTCCCATGTACTCTG25270.8409289819335044No Hit
CATGGGAAGCAGTGGTATCAACGCA23990.7983334498054915No Hit
CAGTGGTATCAACGCAGAGTACATG23950.797002339426491No Hit
GGGAAGCAGTGGTATCAACGCAGAG23640.786686233989238No Hit
CTGCTTCCCATGTACTCTGCGTTGA21900.7287829325027205No Hit
GCAGAGTACATGGGAAGCAGTGGTA21050.7004968369489619No Hit
GCAGTGGTATCAACGCAGAGTACAT20950.6971690610014609No Hit
TACCACTGCTTCCCATGTACTCTGC20500.6821940692377064No Hit
ACATGGGAAGCAGTGGTATCAACGC19900.6622274135527003No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19100.6356052059726923No Hit
CCACTGCTTCCCATGTACTCTGCGT17840.5936752290341796No Hit
CTTCCCATGTACTCTGCGTTGATAC17800.5923441186551792No Hit
GTTGATACCACTGCTTCCCATGTAC17660.5876852323286778No Hit
CCATGTACTCTGCGTTGATACCACT17090.5687169094279221No Hit
AAGCAGTGGTATCAACGCAGAGTAC15650.5207969357839075No Hit
ATGGGAAGCAGTGGTATCAACGCAG15170.5048236112359027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA14730.49018139706689834No Hit
ATCAACGCAGAGTACATGGGAAGCA12580.41863421419562663No Hit
GTACATGGTAAGCAGTGGTATCAAC12490.4156392158428757No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11950.3976692257263703No Hit
TTCCCATGTACTCTGCGTTGATACC11950.3976692257263703No Hit
TGATACCACTGCTTCCCATGTACTC11870.3950070049683695No Hit
GTGGTATCAACGCAGAGTACTTTTT11850.3943414497788693No Hit
ACCATGTACTCTGCGTTGATACCAC9780.3254564876655984No Hit
GGAAGCAGTGGTATCAACGCAGAGT8950.2978359473013401No Hit
ATCAACGCAGAGTACTTTTTTTTTT8340.27753651402158397No Hit
AAAAAGTACTCTGCGTTGATACCAC6910.22994931797231957No Hit
TCAACGCAGAGTACATGGGAAGCAG6560.21830210215606602No Hit
CTGCGTTGATACCACTGCTTCCCAT6420.21364321582956464No Hit
TGGGAAGCAGTGGTATCAACGCAGA6130.2039926655818117No Hit
GGTAAGCAGTGGTATCAACGCAGAG6010.1999993344448105No Hit
CTCTGCGTTGATACCACTGCTTCCC5800.19301100495505838No Hit
CCCCATGTACTCTGCGTTGATACCA5520.18369323230205556No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA5340.17770323559655377No Hit
TCCATGTACTCTGCGTTGATACCAC5340.17770323559655377No Hit
TCCCATGTACTCTGCGTTGATACCA5300.17637212521755335No Hit
AAAGTACTCTGCGTTGATACCACTG4910.16339379902229945No Hit
GTACATGGAAAGCAGTGGTATCAAC4740.15773657991154771No Hit
AACGCAGAGTACATGGGAAGCAGTG4530.1507482504217956No Hit
AGTGGTATCAACGCAGAGTACATGG4410.1467549192847944No Hit
GAGTACATGGTAAGCAGTGGTATCA4370.14542380890579398No Hit
GTACATGGGTAAGCAGTGGTATCAA4360.1450910313110439No Hit
AACGCAGAGTACTTTTTTTTTTTTT4220.14043214498454248No Hit
GCTTACCATGTACTCTGCGTTGATA4190.1394338122002922No Hit
CAACGCAGAGTACTTTTTTTTTTTT4070.13544048106329099No Hit
ACTGCTTCCCATGTACTCTGCGTTG4040.13444214827904066No Hit
ATGTACTCTGCGTTGATACCACTGC4000.13311103790004025No Hit
TTGATACCACTGCTTCCCATGTACT3850.12811937397878875No Hit
CAACGCAGAGTACATGGGAAGCAGT3600.11979993411003623No Hit
GCGTTGATACCACTGCTTACCATGT3550.11813604613628574No Hit
ACTCTGCGTTGATACCACTGCTTAC3500.11647215816253523No Hit
CGCAGAGTACATGGGAAGCAGTGGT3450.11480827018878473No Hit
GAGTACATGGAAAGCAGTGGTATCA3400.11314438221503424No Hit
TCAACGCAGAGTACTTTTTTTTTTT3330.11081493905178352No Hit
CTGCTTACCATGTACTCTGCGTTGA3180.10582327513053201No Hit
ACGCAGAGTACATGGTAAGCAGTGG3050.10149716639878069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1400.014.2581171
AGAGAAC551.9507231E-413.8191495
TACAGGG604.0611447E-412.6717712
GCAGAGA600.005853931411.084113
ACTATCG600.005861190611.08226611
TCGGTCA600.005861190611.08226615
GAGAAAA803.731928E-410.6935881
TCACTAT803.7549748E-410.6864719
AAAAGTA1105.9629874E-610.3678132
TGGTTCA750.002635424310.1340445
CAGAGAA750.002635424310.1340444
CTATCGG856.5658503E-410.05785512
TATCGGT856.5658503E-410.05785513
GGGTAAG951.6354173E-49.9990377
GTACTGG800.0044764879.5054121
ATCGGTC900.00110899619.49908514
AAGTACT1252.727538E-59.1206394
AAAAAGT1304.3014938E-58.7742261
AGTACTC1401.0292334E-48.1434285
AACATGG1200.00168218567.9159049
Produced by FastQC (version 0.11.5)