##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 300501 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502071540527318 33.0 31.0 34.0 28.0 34.0 2 31.57095650264059 33.0 31.0 34.0 28.0 34.0 3 31.67644700017637 33.0 31.0 34.0 28.0 34.0 4 35.0809647888027 37.0 35.0 37.0 32.0 37.0 5 35.39526324371633 37.0 35.0 37.0 33.0 37.0 6 35.122844849102 37.0 35.0 37.0 32.0 37.0 7 35.13356694320485 37.0 35.0 37.0 32.0 37.0 8 35.11815268501602 37.0 35.0 37.0 32.0 37.0 9 36.64577488926825 39.0 37.0 39.0 32.0 39.0 10 36.753388507858546 39.0 37.0 39.0 32.0 39.0 11 36.737361938895376 39.0 37.0 39.0 32.0 39.0 12 36.790965753857726 39.0 37.0 39.0 32.0 39.0 13 36.56200478534181 39.0 37.0 39.0 32.0 39.0 14 37.74972795431629 40.0 38.0 41.0 32.0 41.0 15 37.66586800043927 40.0 38.0 41.0 32.0 41.0 16 37.45825471462658 40.0 37.0 41.0 32.0 41.0 17 37.73968139873079 40.0 38.0 41.0 32.0 41.0 18 37.742037464101614 40.0 38.0 41.0 32.0 41.0 19 37.72869308255214 40.0 38.0 41.0 32.0 41.0 20 37.838160272345185 40.0 38.0 41.0 32.0 41.0 21 37.77083936492724 40.0 38.0 41.0 32.0 41.0 22 37.891670909581 40.0 38.0 41.0 32.0 41.0 23 37.8099773378458 40.0 38.0 41.0 32.0 41.0 24 37.84705208967691 40.0 38.0 41.0 32.0 41.0 25 36.72957494317823 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3335137817035161 1101 2 -0.5025994210752529 1101 3 -0.22245580384232966 1101 4 -0.48390556271176877 1101 5 0.027227997270905746 1101 6 -0.6681744707273438 1101 7 -0.33289272766378275 1101 8 -0.4659734510837765 1101 9 -0.2820046101583742 1101 10 -0.19646776976419034 1101 11 -0.08664826633767575 1101 12 -0.1612012653311794 1101 13 -0.14788667746105233 1101 14 -0.1931144409604002 1101 15 -0.2468360228906903 1101 16 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-0.3103612712714252 1103 2 -0.16832728588778778 1103 3 -0.00796891773072872 1103 4 -0.13311810814184355 1103 5 -0.11533700267732172 1103 6 -0.21579450448279403 1103 7 0.025054816782613898 1103 8 0.04503331036045921 1103 9 -0.02107809556380147 1103 10 -0.048678958763638036 1103 11 -0.23861509073685738 1103 12 -0.14517778147891391 1103 13 0.01047918604682252 1103 14 -0.03177685540690334 1103 15 -0.19899336884262198 1103 16 0.14637741028066387 1103 17 0.24644823629069634 1103 18 -0.08307749960183486 1103 19 -0.05728414268997284 1103 20 -0.26383902761531175 1103 21 -0.10558352465209708 1103 22 -0.03174977971662685 1103 23 -0.30076388071002924 1103 24 -0.21346450554416663 1103 25 -0.009930689499107359 1104 1 -0.19658242640014123 1104 2 -0.2150741897775461 1104 3 -0.06544318949071481 1104 4 -0.3123898168253163 1104 5 -0.0036359119846309795 1104 6 -0.39978091956164263 1104 7 -0.2603978258128805 1104 8 -0.43537936753121187 1104 9 -0.1580508333768762 1104 10 -0.23080309046760306 1104 11 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1107 7 -0.031007764703694818 1107 8 -0.12899268856583745 1107 9 -0.20932105898124576 1107 10 -0.15566569087499715 1107 11 -0.19085452627638944 1107 12 0.007393351276668625 1107 13 -0.15427654675477953 1107 14 0.09328066434154181 1107 15 -0.12178269317730894 1107 16 -0.1025872342472951 1107 17 -0.1959840821386294 1107 18 -0.19759931875974956 1107 19 -0.21181094689275426 1107 20 -0.11442189439956962 1107 21 -0.0975115866200369 1107 22 0.07846764493250902 1107 23 0.07470586390228817 1107 24 0.18519528431701815 1107 25 0.025789074286457492 1108 1 -0.03597715865201678 1108 2 0.004749637189135569 1108 3 -0.005832165593975702 1108 4 -0.1960894505249442 1108 5 0.20140507877064806 1108 6 -0.16752904445262828 1108 7 -5.862088584080993E-4 1108 8 -0.1592150556526164 1108 9 -0.1246477285452059 1108 10 0.1035332081544098 1108 11 -0.088036910746915 1108 12 0.15698410891827308 1108 13 -0.009882804903405429 1108 14 0.2798118826524245 1108 15 0.35467681718050414 1108 16 -0.24288855251471375 1108 17 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25 -0.2050539677297749 2215 1 0.3893770379787753 2215 2 0.33680587881984536 2215 3 0.3072371527944924 2215 4 0.3048156727206006 2215 5 0.2743852900487269 2215 6 0.48507721453660935 2215 7 0.46092745478291164 2215 8 0.4041229066215166 2215 9 0.5405723941217317 2215 10 0.40152540508277923 2215 11 0.3433142440084964 2215 12 0.4216190012127399 2215 13 0.43231329686716435 2215 14 0.45094630378684286 2215 15 0.5062456974727922 2215 16 0.4311389566191792 2215 17 0.42716364103613813 2215 18 0.40989010850969976 2215 19 0.276677890270804 2215 20 0.4388925336894687 2215 21 0.3789907246280393 2215 22 0.46062267886359365 2215 23 0.5068846659873287 2215 24 0.41693888330728157 2215 25 0.4062188680772323 2216 1 0.3135633974102774 2216 2 0.14863835716481333 2216 3 0.057552517355922816 2216 4 0.263650085301542 2216 5 0.1679602627171306 2216 6 0.41035683778108023 2216 7 0.22785201957982082 2216 8 0.34575943845231194 2216 9 0.23567793612829036 2216 10 0.33920936774361365 2216 11 0.342725563493822 2216 12 0.19598616965946292 2216 13 0.1729995301528433 2216 14 0.2651755527987021 2216 15 0.33897633408871997 2216 16 0.5824298488906479 2216 17 0.23584247376677325 2216 18 0.404045351971142 2216 19 0.18105935524431516 2216 20 0.3053041054664405 2216 21 0.24657965743435994 2216 22 0.3155129319962171 2216 23 0.18067156364102033 2216 24 0.247188652039938 2216 25 -0.1538685930278021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 13.0 9 63.0 10 113.0 11 157.0 12 224.0 13 298.0 14 519.0 15 873.0 16 1228.0 17 1461.0 18 1579.0 19 1616.0 20 1618.0 21 1584.0 22 1362.0 23 1386.0 24 1359.0 25 1438.0 26 1475.0 27 1585.0 28 1781.0 29 2158.0 30 2665.0 31 3599.0 32 4979.0 33 7021.0 34 10236.0 35 15814.0 36 26450.0 37 54457.0 38 151390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.21545364970307 18.50782935208117 12.209062341881708 22.067654656334053 2 12.602642151266322 24.74096066729464 33.89396112995403 28.762436051485 3 22.461380774580864 32.15761835353314 27.48104812677622 17.899952745109786 4 13.514763677991088 23.616227566630393 27.759974176458645 35.109034578919875 5 10.139400534440817 40.29936672423719 34.48075048003168 15.080482261290312 6 31.07639466695067 29.898890390260462 18.169110648126576 20.855604294662292 7 19.398937108362368 37.27009227922702 23.16797614650201 20.1629944659086 8 29.28775611395636 31.708047560573842 21.22522054835092 17.778975777118877 9 27.971953504314463 16.453522617229225 20.287786063939887 35.28673781451642 10 19.722064152864714 32.03184016026569 28.56030429183264 19.685791395036954 11 24.82088245962576 27.745997517479143 17.525399249919303 29.9077207729758 12 25.947667395449596 34.02617628560304 26.561642057763535 13.464514261183824 13 31.974935191563425 20.821228548324296 22.91340128651818 24.290434973594095 14 22.81090578733515 29.466457682337165 26.125370631046152 21.597265899281535 15 36.97758077344169 19.137041141294038 24.15399615974656 19.73138192551772 16 15.443209839567922 27.25015890130149 36.89538470753841 20.411246551592175 17 25.159317273486607 29.746323639522 28.121037866762506 16.973321220228886 18 25.304741082392407 20.27380940496038 33.766942539292714 20.654506973354497 19 21.873471302924116 29.95331130345656 35.491063257692986 12.682154135926336 20 23.519722064152866 22.305083843314996 33.304381682590076 20.87081240994206 21 18.04719451848746 27.108062868343204 36.46310661195803 18.38163600121131 22 21.923387942136632 25.636853121953006 26.226202242255432 26.213556693654926 23 16.057184501881856 25.97795015657186 34.22983617359011 23.735029167956178 24 20.375972126548664 33.78358141903022 28.72902253237094 17.111423922050175 25 20.807251889344794 22.023221220561663 27.37428494414328 29.795241945950263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1852.0 1 1852.0 2 1626.0 3 1400.0 4 1400.0 5 1400.0 6 2689.0 7 3978.0 8 3978.0 9 3978.0 10 3663.5 11 3349.0 12 3349.0 13 3349.0 14 2560.0 15 1771.0 16 1771.0 17 1771.0 18 2656.5 19 3542.0 20 3542.0 21 3542.0 22 5470.5 23 7399.0 24 7399.0 25 7399.0 26 9586.5 27 11774.0 28 11774.0 29 11774.0 30 12942.5 31 14111.0 32 14111.0 33 14111.0 34 14186.0 35 14261.0 36 14261.0 37 14261.0 38 12784.0 39 11307.0 40 11307.0 41 11307.0 42 15350.0 43 19393.0 44 19393.0 45 19393.0 46 58560.5 47 97728.0 48 97728.0 49 97728.0 50 82329.5 51 66931.0 52 66931.0 53 66931.0 54 45050.0 55 23169.0 56 23169.0 57 23169.0 58 15438.5 59 7708.0 60 7708.0 61 7708.0 62 6493.0 63 5278.0 64 5278.0 65 5278.0 66 4103.5 67 2929.0 68 2929.0 69 2929.0 70 2272.0 71 1615.0 72 1615.0 73 1615.0 74 1129.0 75 643.0 76 643.0 77 643.0 78 451.5 79 260.0 80 260.0 81 260.0 82 163.5 83 67.0 84 67.0 85 67.0 86 48.5 87 30.0 88 30.0 89 30.0 90 17.5 91 5.0 92 5.0 93 5.0 94 3.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030948316311759365 2 0.02129776606400644 3 9.98332784250302E-4 4 0.0 5 0.0 6 0.011647215816253524 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 300501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.23153333932333 #Duplication Level Percentage of deduplicated Percentage of total 1 78.46627114919798 19.01358065364175 2 12.289881344759394 5.956053390837301 3 4.081520544935179 2.9670450347918975 4 1.8402548890353767 1.7836879078605397 5 0.9297407163260822 1.1264521582290907 6 0.4834102395078005 0.7028262801122126 7 0.3323445396616128 0.5637252455066706 8 0.24170511975390024 0.4685508534081418 9 0.13595912986156888 0.2965048369223397 >10 0.9146341463414633 4.348072053004815 >50 0.1043726653482751 1.7730390248285364 >100 0.1043726653482751 5.727767960838732 >500 0.01785321907273127 2.8751984186408697 >1k 0.0453197099538563 25.86879910549382 >5k 0.00961327180839376 14.929401233273767 >10k+ 0.002746649088112503 11.599295842609509 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 17809 5.926436184904542 No Hit GTACATGGGAAGCAGTGGTATCAAC 17047 5.672859657704966 No Hit GAGTACATGGGAAGCAGTGGTATCA 7999 2.661887980406055 No Hit GTATCAACGCAGAGTACTTTTTTTT 7340 2.442587545465739 No Hit CATGTACTCTGCGTTGATACCACTG 6712 2.2336032159626753 No Hit GGTATCAACGCAGAGTACTTTTTTT 6166 2.0519066492291205 No Hit GCGTTGATACCACTGCTTCCCATGT 5778 1.9227889424660816 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5756 1.9154678353815795 No Hit TATCAACGCAGAGTACATGGGAAGC 5112 1.7011590643625145 No Hit GGTATCAACGCAGAGTACATGGGAA 4936 1.6425902076864969 No Hit GTATCAACGCAGAGTACATGGGAAG 4893 1.6282807711122425 No Hit TATCAACGCAGAGTACTTTTTTTTT 4833 1.6083141154272365 No Hit ACTCTGCGTTGATACCACTGCTTCC 4569 1.52046083041321 No Hit GCTTCCCATGTACTCTGCGTTGATA 3368 1.1207949391183392 No Hit GTGGTATCAACGCAGAGTACATGGG 3130 1.041593871567815 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3051 1.0153044415825572 No Hit GTACTCTGCGTTGATACCACTGCTT 2866 0.9537405865537885 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2647 0.8808622933035165 No Hit GATACCACTGCTTCCCATGTACTCT 2555 0.8502467545865071 No Hit ATACCACTGCTTCCCATGTACTCTG 2527 0.8409289819335044 No Hit CATGGGAAGCAGTGGTATCAACGCA 2399 0.7983334498054915 No Hit CAGTGGTATCAACGCAGAGTACATG 2395 0.797002339426491 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2364 0.786686233989238 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2190 0.7287829325027205 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2105 0.7004968369489619 No Hit GCAGTGGTATCAACGCAGAGTACAT 2095 0.6971690610014609 No Hit TACCACTGCTTCCCATGTACTCTGC 2050 0.6821940692377064 No Hit ACATGGGAAGCAGTGGTATCAACGC 1990 0.6622274135527003 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1910 0.6356052059726923 No Hit CCACTGCTTCCCATGTACTCTGCGT 1784 0.5936752290341796 No Hit CTTCCCATGTACTCTGCGTTGATAC 1780 0.5923441186551792 No Hit GTTGATACCACTGCTTCCCATGTAC 1766 0.5876852323286778 No Hit CCATGTACTCTGCGTTGATACCACT 1709 0.5687169094279221 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1565 0.5207969357839075 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1517 0.5048236112359027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1473 0.49018139706689834 No Hit ATCAACGCAGAGTACATGGGAAGCA 1258 0.41863421419562663 No Hit GTACATGGTAAGCAGTGGTATCAAC 1249 0.4156392158428757 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1195 0.3976692257263703 No Hit TTCCCATGTACTCTGCGTTGATACC 1195 0.3976692257263703 No Hit TGATACCACTGCTTCCCATGTACTC 1187 0.3950070049683695 No Hit GTGGTATCAACGCAGAGTACTTTTT 1185 0.3943414497788693 No Hit ACCATGTACTCTGCGTTGATACCAC 978 0.3254564876655984 No Hit GGAAGCAGTGGTATCAACGCAGAGT 895 0.2978359473013401 No Hit ATCAACGCAGAGTACTTTTTTTTTT 834 0.27753651402158397 No Hit AAAAAGTACTCTGCGTTGATACCAC 691 0.22994931797231957 No Hit TCAACGCAGAGTACATGGGAAGCAG 656 0.21830210215606602 No Hit CTGCGTTGATACCACTGCTTCCCAT 642 0.21364321582956464 No Hit TGGGAAGCAGTGGTATCAACGCAGA 613 0.2039926655818117 No Hit GGTAAGCAGTGGTATCAACGCAGAG 601 0.1999993344448105 No Hit CTCTGCGTTGATACCACTGCTTCCC 580 0.19301100495505838 No Hit CCCCATGTACTCTGCGTTGATACCA 552 0.18369323230205556 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 534 0.17770323559655377 No Hit TCCATGTACTCTGCGTTGATACCAC 534 0.17770323559655377 No Hit TCCCATGTACTCTGCGTTGATACCA 530 0.17637212521755335 No Hit AAAGTACTCTGCGTTGATACCACTG 491 0.16339379902229945 No Hit GTACATGGAAAGCAGTGGTATCAAC 474 0.15773657991154771 No Hit AACGCAGAGTACATGGGAAGCAGTG 453 0.1507482504217956 No Hit AGTGGTATCAACGCAGAGTACATGG 441 0.1467549192847944 No Hit GAGTACATGGTAAGCAGTGGTATCA 437 0.14542380890579398 No Hit GTACATGGGTAAGCAGTGGTATCAA 436 0.1450910313110439 No Hit AACGCAGAGTACTTTTTTTTTTTTT 422 0.14043214498454248 No Hit GCTTACCATGTACTCTGCGTTGATA 419 0.1394338122002922 No Hit CAACGCAGAGTACTTTTTTTTTTTT 407 0.13544048106329099 No Hit ACTGCTTCCCATGTACTCTGCGTTG 404 0.13444214827904066 No Hit ATGTACTCTGCGTTGATACCACTGC 400 0.13311103790004025 No Hit TTGATACCACTGCTTCCCATGTACT 385 0.12811937397878875 No Hit CAACGCAGAGTACATGGGAAGCAGT 360 0.11979993411003623 No Hit GCGTTGATACCACTGCTTACCATGT 355 0.11813604613628574 No Hit ACTCTGCGTTGATACCACTGCTTAC 350 0.11647215816253523 No Hit CGCAGAGTACATGGGAAGCAGTGGT 345 0.11480827018878473 No Hit GAGTACATGGAAAGCAGTGGTATCA 340 0.11314438221503424 No Hit TCAACGCAGAGTACTTTTTTTTTTT 333 0.11081493905178352 No Hit CTGCTTACCATGTACTCTGCGTTGA 318 0.10582327513053201 No Hit ACGCAGAGTACATGGTAAGCAGTGG 305 0.10149716639878069 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 3.3277759475010065E-4 0.0 13 0.0 0.0 0.0 3.3277759475010065E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 140 0.0 14.258117 1 AGAGAAC 55 1.9507231E-4 13.819149 5 TACAGGG 60 4.0611447E-4 12.671771 2 GCAGAGA 60 0.0058539314 11.08411 3 ACTATCG 60 0.0058611906 11.082266 11 TCGGTCA 60 0.0058611906 11.082266 15 GAGAAAA 80 3.731928E-4 10.693588 1 TCACTAT 80 3.7549748E-4 10.686471 9 AAAAGTA 110 5.9629874E-6 10.367813 2 TGGTTCA 75 0.0026354243 10.134044 5 CAGAGAA 75 0.0026354243 10.134044 4 CTATCGG 85 6.5658503E-4 10.057855 12 TATCGGT 85 6.5658503E-4 10.057855 13 GGGTAAG 95 1.6354173E-4 9.999037 7 GTACTGG 80 0.004476487 9.505412 1 ATCGGTC 90 0.0011089961 9.499085 14 AAGTACT 125 2.727538E-5 9.120639 4 AAAAAGT 130 4.3014938E-5 8.774226 1 AGTACTC 140 1.0292334E-4 8.143428 5 AACATGG 120 0.0016821856 7.915904 9 >>END_MODULE