FastQCFastQC Report
Wed 25 May 2016
SRR1294926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699005
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC273731.6111194493247518No Hit
CCCATGTACTCTGCGTTGATACCAC254281.496640680869097No Hit
GAGTACATGGGAAGCAGTGGTATCA119050.7007042357144329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA107360.6318992586837591No Hit
CATGTACTCTGCGTTGATACCACTG93030.5475557752920092No Hit
GCGTTGATACCACTGCTTCCCATGT86000.5061786163077802No Hit
GTATCAACGCAGAGTACATGGGAAG76600.45085211638576694No Hit
TATCAACGCAGAGTACATGGGAAGC73250.4311346935412197No Hit
ACGCAGAGTACATGGGAAGCAGTGG71940.4234242983393221No Hit
GGTATCAACGCAGAGTACATGGGAA68880.4054137568753476No Hit
ACTCTGCGTTGATACCACTGCTTCC60710.35732678832610854No Hit
GTATCAACGCAGAGTACTTTTTTTT48630.28622635012845754No Hit
GCTTCCCATGTACTCTGCGTTGATA46770.2752787661013358No Hit
GTGGTATCAACGCAGAGTACATGGG46610.27433703844308877No Hit
GTACTCTGCGTTGATACCACTGCTT40070.23584392041224128No Hit
ATACCACTGCTTCCCATGTACTCTG34790.2047669076900892No Hit
GGTATCAACGCAGAGTACTTTTTTT32890.19358389174840568No Hit
GGGAAGCAGTGGTATCAACGCAGAG31500.18540263271738458No Hit
CAGTGGTATCAACGCAGAGTACATG31340.18446090505913756No Hit
GCAGTGGTATCAACGCAGAGTACAT31250.18393118325137361No Hit
GATACCACTGCTTCCCATGTACTCT30150.17745680560092525No Hit
CTGCTTCCCATGTACTCTGCGTTGA29690.17474933858346503No Hit
GCAGAGTACATGGGAAGCAGTGGTA29290.17239501943784744No Hit
TACCACTGCTTCCCATGTACTCTGC28630.16851039284757843No Hit
CATGGGAAGCAGTGGTATCAACGCA28470.16756866518933142No Hit
TATCAACGCAGAGTACTTTTTTTTT27060.15926969020102943No Hit
GTTGATACCACTGCTTCCCATGTAC25800.15185358489233403No Hit
ACATGGGAAGCAGTGGTATCAACGC24840.14620321894285185No Hit
CCATGTACTCTGCGTTGATACCACT23350.13743338012542636No Hit
CCACTGCTTCCCATGTACTCTGCGT23340.13737452214678592No Hit
CTTCCCATGTACTCTGCGTTGATAC23190.13649165246717934No Hit
AAGCAGTGGTATCAACGCAGAGTAC20770.12224802163619294No Hit
ATGGGAAGCAGTGGTATCAACGCAG19710.11600907590030636No Hit
TGATACCACTGCTTCCCATGTACTC17690.10411976421493756No Hit
ATCAACGCAGAGTACATGGGAAGCA17320.1019420190052413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAC853.938794E-612.29554753
AGCGTCA550.00307206812.08910910
CTTAGAC658.0162653E-411.6936673
GTAACAC909.504129E-510.5567843
GTATAGC750.002623971810.1443751
GGACCGC750.002652353810.13122756
GTAAAAA4650.010.0216611
GTCTTAT1856.348273E-109.7673881
TCAGGAC1401.0565764E-69.5011053
TAATAGA1701.6607373E-89.5011044
TGCGCAG900.00111514179.49858610
TTATACT2550.09.3148094
GCGCAGA1054.4986844E-49.04627211
GACTGTC850.0074480358.9393197
TATACTG2607.2759576E-128.7689595
GTCATAG1201.7427324E-48.7178221
GAGAGCT1554.2169213E-68.5788637
GCCTTAA1000.0028762028.5593161
GTTATAC1000.00290164288.5509943
GACTGAT1000.0029101678.5482247
Produced by FastQC (version 0.11.5)