FastQCFastQC Report
Wed 25 May 2016
SRR1294926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699005
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC280281.64967142533424No Hit
GTACATGGGAAGCAGTGGTATCAAC269861.5883414115909016No Hit
GTATCAACGCAGAGTACTTTTTTTT159790.9404916406955837No Hit
GAGTACATGGGAAGCAGTGGTATCA120660.7101803702755437No Hit
GGTATCAACGCAGAGTACTTTTTTT113300.6668608979961801No Hit
CATGTACTCTGCGTTGATACCACTG99540.5858723193869353No Hit
TATCAACGCAGAGTACTTTTTTTTT97620.5745715874879709No Hit
GCGTTGATACCACTGCTTCCCATGT91080.5360784694571235No Hit
ACGCAGAGTACATGGGAAGCAGTGG77650.4570322041430131No Hit
GTATCAACGCAGAGTACATGGGAAG76320.4492040929838346No Hit
TATCAACGCAGAGTACATGGGAAGC73990.43549018396061223No Hit
GGTATCAACGCAGAGTACATGGGAA67720.39858623135305665No Hit
ACTCTGCGTTGATACCACTGCTTCC65610.3861671978599239No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50400.2966442123478153No Hit
GCTTCCCATGTACTCTGCGTTGATA49000.2884040953381538No Hit
GTGGTATCAACGCAGAGTACATGGG46970.27645592567414456No Hit
GTACTTTTTTTTTTTTTTTTTTTTT42390.24949897145682326No Hit
GTACTCTGCGTTGATACCACTGCTT40430.2379628076432971No Hit
ATACCACTGCTTCCCATGTACTCTG37920.2231894550045468No Hit
CAGTGGTATCAACGCAGAGTACATG33680.19823367206100043No Hit
GGGAAGCAGTGGTATCAACGCAGAG32840.1932896018552035No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32420.19081756675230502No Hit
GATACCACTGCTTCCCATGTACTCT31800.18716837207659778No Hit
CTGCTTCCCATGTACTCTGCGTTGA31780.18705065611931687No Hit
CATGGGAAGCAGTGGTATCAACGCA31150.1833426034649692No Hit
TACCACTGCTTCCCATGTACTCTGC30040.17680936783588042No Hit
GCAGAGTACATGGGAAGCAGTGGTA28280.16645036359516305No Hit
GCAGTGGTATCAACGCAGAGTACAT28240.1662149316806013No Hit
ACATGGGAAGCAGTGGTATCAACGC26660.15691537105541184No Hit
CCATGTACTCTGCGTTGATACCACT25610.15073528329816568No Hit
CCACTGCTTCCCATGTACTCTGCGT25470.14991127159719952No Hit
GTTGATACCACTGCTTCCCATGTAC24880.1464386508574136No Hit
CTTCCCATGTACTCTGCGTTGATAC24810.14602664500693052No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20910.1230720333371591No Hit
AAGCAGTGGTATCAACGCAGAGTAC20880.12289545940123779No Hit
ATGGGAAGCAGTGGTATCAACGCAG20220.11901083281096878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18460.10865182857025141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA456.760644E-414.7783964
CGGACAG550.003067687412.0914145
GTCTTAG802.8482851E-511.8824921
AGTCCGT802.8689557E-511.87409910
CCCCTAG657.9817534E-411.6996841
CTAGGGC700.001491005910.8575973
CTAGACT909.51132E-510.5559974
TGCGCCA750.002649456710.13256512
GTATAGG1054.0764753E-59.9586581
GTGTAAG1356.3859807E-79.8580671
CTTGGAC1304.235362E-69.5003983
GTTATTC1201.6992926E-59.5003973
ATAAGGC1554.2559623E-79.1939333
GTATTAG1252.7330672E-59.1257531
TATGCTG1151.1099425E-49.0873375
GTATTAC850.0073969928.9468171
ACGCAAA850.0073969928.9468171
GACTTAG850.0073969928.9468171
GTAGCAC850.0074328078.941553
TGCCGTG850.00743998778.9404987
Produced by FastQC (version 0.11.5)