FastQCFastQC Report
Wed 25 May 2016
SRR1294925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708464
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA34830.4916269563449942No Hit
GTATCAACGCAGAGTACTTTTTTTT17120.24164954041419182No Hit
GGTATCAACGCAGAGTACTTTTTTT14280.2015628175884731No Hit
GGTATCAACGCAGAGTACATGGGGG10780.1521601662187493No Hit
TATCAACGCAGAGTACTTTTTTTTT10370.14637299848686736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC350.00216626116.2883783
GGTATCA6050.014.3077661
GTATTGG551.9363932E-413.83608151
CCTACAC500.001496294613.3021763
GTATGGA604.0427342E-412.6830751
GTGTAGG751.4563722E-512.6830751
GTGGTAT1700.012.3100441
GTGTAAA550.003036022412.1065711
CCCATAA550.00306996512.0886168
GTCTTAG657.9273735E-411.7074541
TGGTATC1604.5474735E-1111.2830952
GACTTGT600.00587859511.0812327
TAAGGCT909.481634E-510.5572824
GCACAGT909.515653E-510.5535546
TGTAGGA1002.3889941E-510.451712
GTGTAGA750.00261627110.146461
GTGTATG1151.00797715E-59.9258841
GTAAAAA1151.00797715E-59.9258841
ATACTGT1151.0259208E-59.9111646
GTGTACA800.0044624029.5123061
Produced by FastQC (version 0.11.5)