FastQCFastQC Report
Wed 25 May 2016
SRR1294925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708464
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59310.8371632150680909No Hit
GGTATCAACGCAGAGTACTTTTTTT47050.6641127848415728No Hit
TATCAACGCAGAGTACTTTTTTTTT35490.5009428848889993No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19390.27369068858826984No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11720.16542830687233226No Hit
GGTATCAACGCAGAGTACATGGGGG10990.1551243253009327No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9620.13578671605049797No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7520.1061451252286637No Hit
GTGGTATCAACGCAGAGTACTTTTT7450.10515707220126921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9750.014.9125671
TAGACAG653.3667784E-614.6150055
TAACCCA550.003066210812.0905944
CTAGACA658.015719E-411.6920044
GTGGTAT2300.011.1558211
ACCATCG600.005871447311.0830458
ATACTAT600.005871447311.0830456
TGGTATC2150.011.0477832
CCTTAAT951.3522809E-511.0035961
CAAACCT750.002645101910.133074
TCTAGAC750.002645101910.133073
TTTATAG750.002645101910.133073
GGATAGG856.5637496E-410.0621131
TTGAGAC1252.562494E-69.8797443
TACACTG1252.562494E-69.8797445
GTGTTAT800.00449818149.5031061
GTACAAG1106.7962006E-59.5031061
GTATCAA18500.09.143531
TACTGTG1054.4849524E-49.0473847
GTCTATA950.00181550079.0029431
Produced by FastQC (version 0.11.5)