FastQCFastQC Report
Wed 25 May 2016
SRR1294924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522602
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC97610.21582708361248681No Hit
CCCATGTACTCTGCGTTGATACCAC92560.20466094518155697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCC1201.5294809E-610.2896539
GTATTAA8100.09.5147751
GCTCGAC800.00452473859.49835111
ACACCGT2002.6557245E-109.49793156
TAGACAG6050.09.4214155
CCGTCCA1151.1139078E-49.0851789
TCGGGAC850.0074310278.9423953
TACACCG1606.750088E-78.9061825
GTACTAG3850.08.8969331
GTCCTAC3250.08.7828691
GCTAGAC2607.2759576E-128.7704273
TGGATCG1304.372979E-58.7691645
CCTACAC5650.08.7445553
GTTCTAG5100.08.5819531
CTACACT5800.08.3544954
GTATAAA9650.08.2822911
GTATAGG5450.08.2054021
CTAGACT5350.08.1692484
GTATTAG7350.08.1555211
GTCCTAG6100.08.1109551
Produced by FastQC (version 0.11.5)