FastQCFastQC Report
Wed 25 May 2016
SRR1294924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522602
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC100000.22111165209761993No Hit
GTACATGGGAAGCAGTGGTATCAAC98280.21730853168154085No Hit
GTATCAACGCAGAGTACTTTTTTTT97390.21534063797787203No Hit
GGTATCAACGCAGAGTACTTTTTTT60880.134612773797031No Hit
TATCAACGCAGAGTACTTTTTTTTT55360.12240741060124238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3450.09.6380184
ACCGTTC1002.759855E-49.4991768
TTACGCT1054.4941012E-49.04793554
AGACCCG950.0018242819.0003155
GTCTTAG4000.08.7934461
TCTATAC6000.08.709123
TTAGACA5600.08.6520874
CATCGTA1000.00290890538.5492589
GTCTAGT3150.08.4501581
ACACGAC1356.7965164E-58.4452073
CGCCCGC1804.0444866E-78.44371310
GTAGGAC3200.08.3132513
CTAGGAC4700.08.2879813
GTCTAAG3700.08.2217761
CTAGTAG3850.08.1435923
ACACCGT1752.4412439E-68.1431416
CACGACT1401.0395122E-48.1431414
CACCGTA1050.004518828.1421527
CCTATAC4450.08.11309053
TTATACT8450.08.0949574
Produced by FastQC (version 0.11.5)