FastQCFastQC Report
Wed 25 May 2016
SRR1294923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177243
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1103.812238E-812.0927394
GGTCGAA803.7808085E-410.68527611
ATACGGT1451.625449E-79.825076
GTATCAA27050.09.7422461
CGAGTAA800.004525019.49825110
GTATTAG7300.09.1226651
TACACCG950.0018244749.0001765
GGGTCGA950.0018278588.99834310
ACCGTGT2553.6379788E-128.9391028
TTATACG1401.0840493E-58.8227644
CTAGGAC4450.08.7540293
TACCCTG5150.08.6700735
GTGTAAG6900.08.5484851
GTATTAT10350.08.4565661
TATACGG1252.7445992E-48.3601635
AATACGG1252.7445992E-48.3601635
CTGTGCG1252.7520637E-48.358069
TTAGACA5700.08.3345954
CTTAGAC4450.08.32700353
GTCTAGA4250.08.282461
Produced by FastQC (version 0.11.5)