FastQCFastQC Report
Wed 25 May 2016
SRR1294923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177243
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84330.20187956506241078No Hit
TATCAACGCAGAGTACTTTTTTTTT47120.11280167325673895No Hit
GGTATCAACGCAGAGTACTTTTTTT45670.10933048424523065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG600.005877287611.0836345
TGAACCG1053.4690547E-610.8574375
CCGTGCA1002.4028552E-510.4492839
AACCGTG1106.056918E-610.3629257
TAGACAG5700.010.1669435
GGTATCA20050.09.9553991
GTATCAA37500.09.5557311
TTACACT7650.09.3139784
GTATCGT950.00182435069.0002436
GTATTAG6200.08.7385011
TATACTG8600.08.726985
GTCGCCC2751.8189894E-128.63577111
TATCGTT1107.1535754E-48.6357717
TACACTG10200.08.5688595
CTAGAAC5350.08.5240043
TAGACTG6300.08.4446735
TGCGTTG6200.08.4268412
GTCTTAC3900.08.2864421
CTAGACA4750.08.2002224
ATAATAC9750.08.1852293
Produced by FastQC (version 0.11.5)