FastQCFastQC Report
Wed 25 May 2016
SRR1294922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978284
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC86220.21672660875895233No Hit
CCCATGTACTCTGCGTTGATACCAC81790.20559115437711337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG400.005286752214.2476699
CTGTGCG951.3640321E-510.9982019
CGAAGAT750.00265050610.13269614
TATATCG1151.02865815E-59.91204555
CGGGACT800.0045171259.5002384
TAGGCCG1201.7039834E-59.4990435
TATACTG7900.09.4990435
ACACCGT1201.7049986E-59.4985666
ACCTCGC1002.7614465E-49.4985668
CTAGGAC4750.09.4002373
GTATTAG6500.09.3659841
TGGACCG1054.4998908E-49.0467085
CGGACTG1054.4998908E-49.0467085
CCGGACT850.00743747278.94140154
TACGTAA850.00743747278.94140152
TAGACAG6650.08.8562525
TTAGACA4400.08.8524954
CTAGTAC2054.145477E-98.80509953
CGTGGAC1107.1467116E-48.636583
CTGTCGC3200.08.6079669
Produced by FastQC (version 0.11.5)