FastQCFastQC Report
Wed 25 May 2016
SRR1294922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978284
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92540.232612855190831No Hit
CCCATGTACTCTGCGTTGATACCAC88810.22323695342011782No Hit
GTACATGGGAAGCAGTGGTATCAAC85550.2150424655454462No Hit
GGTATCAACGCAGAGTACTTTTTTT52370.13163967177808322No Hit
TATCAACGCAGAGTACTTTTTTTTT50110.1259588304907342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG604.0906825E-412.6680755
GCGGAAC856.604745E-410.05766611
GTCCTAG4300.09.507391
TGCGCAT850.00744603768.94014710
GTCTAGC2401.0004442E-108.7151091
TTAGACA4350.08.5181884
TGCGTTG23750.08.39903312
CTGTCGC2951.8189894E-128.3719189
CTAGTAC2855.456968E-128.334263
TAGACTG6050.08.3232395
TATACTG9050.08.1887555
TCTAGAC3250.08.1855253
CTGCGTT24500.08.1419211
TGTCGCC2803.45608E-118.1419210
GTATTAA7250.08.1304581
CTAATAC6100.08.099263
CCAGGAC7300.08.069393
GTATAGA5900.08.0571111
CAGGACT5550.08.0459394
TAGGACC2605.2568794E-108.0393544
Produced by FastQC (version 0.11.5)