FastQCFastQC Report
Wed 25 May 2016
SRR1294921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200501
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44270.10539219012208306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG2300.014.044722
GCCGCGG1950.012.6677873
TTACGCT856.591114E-410.0599534
AAGCCGC1502.5979352E-79.5134261
CCGCGGG1401.0582171E-69.5010674
CGCGGCG1106.8435475E-59.4998215
ACGACTG800.00451885269.4998215
GTACCGA800.00452329649.4986896
GAGCCGC2054.129106E-109.2813911
CCGCGGC1554.2588363E-79.194584
ATTAGAC3100.08.8878823
CTAGGAC4000.08.7882773
TAGACAG5800.08.6808725
TAGGACA6050.08.6373344
TACATCG1000.00290702758.5498395
GTCCTAA3350.08.5194861
CTAGACA5400.08.4453934
ATAGGAC5350.08.3465333
GTCTTAT5600.08.3242471
CTAGCAC4800.08.3132353
Produced by FastQC (version 0.11.5)