FastQCFastQC Report
Wed 25 May 2016
SRR1294921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200501
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114330.2721818183116728No Hit
GGTATCAACGCAGAGTACTTTTTTT72530.17266987914060727No Hit
TATCAACGCAGAGTACTTTTTTTTT64940.15460060597533484No Hit
CCCATGTACTCTGCGTTGATACCAC44640.10627303743053507No Hit
GTACATGGGAAGCAGTGGTATCAAC43690.10401140244937448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGT1002.4029185E-510.449268
GCACCGA856.594999E-410.0593096
ACTGCGT1252.5752215E-69.87938
GTCTTAG4450.09.6115531
CTAGACT3900.09.2569674
GTATACG850.00740866558.9456551
GTATCAA56850.08.6604481
GGTATCA33350.08.5785071
AGGACCG1000.00290526388.5504135
TACACCG1252.7437595E-48.3604035
CTTACAC5300.08.2458773
TTACACT7800.08.1607484
TGTACGC1050.0045131968.143255
CTGCGTG2105.8224032E-88.1422819
CCAGTAC5150.08.117093
ATAGTAC4800.08.1151683
GTCTAGA4600.08.0583821
ACACCGT1304.186187E-48.038856
GTCTTAA4750.08.0040061
TTATACT9400.07.98452234
Produced by FastQC (version 0.11.5)