FastQCFastQC Report
Wed 25 May 2016
SRR1294920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4119062
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC90740.22029287250349716No Hit
CCCATGTACTCTGCGTTGATACCAC87370.21211139817754626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1054.108909E-59.952335
ACTGCGT1252.5774116E-69.8786298
CCGAATT800.00452148039.49914414
GTATTAG6650.09.1531531
GTCCACG850.00737073378.9512461
TTATACT8350.08.6476094
TGACCGA1107.1593735E-48.6350610
GTCTAAT4000.08.5596291
CTTACAC5000.08.5506833
TAGGACT5450.08.5421754
AGCACCG1451.6907345E-58.5171985
GTCTTAC4500.08.4539551
ACTAGAC3150.08.445123
TTGGACT4200.08.369924
GTCTAGG4450.08.3352191
GTATAAA9800.08.2490761
CTAGGAC3700.08.2168733
TAGACTG5600.08.1428155
ACCGTGG1401.04154664E-48.1417278
ACCGTCC1050.0045207638.1417278
Produced by FastQC (version 0.11.5)