FastQCFastQC Report
Wed 25 May 2016
SRR1294920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4119062
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105540.2562233828963973No Hit
CCCATGTACTCTGCGTTGATACCAC93670.22740614246641588No Hit
GTACATGGGAAGCAGTGGTATCAAC88380.21456341273814283No Hit
GGTATCAACGCAGAGTACTTTTTTT66200.1607162018925668No Hit
TATCAACGCAGAGTACTTTTTTTTT58790.14272666932422964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG856.59202E-410.0597935
TACACCG900.00111335259.50091655
GTTCGAT800.00452131179.49918711
ACGCTCA800.00452131179.49918717
CTAGACT3950.09.3806514
CTAGGAC5300.08.9634553
TCTAGAC3400.08.6629153
GCACCGT1000.00290397168.5508256
CGTTCCG1000.0029087628.54926815
AGACCGT1356.795564E-58.4452596
CCAGGAC7050.08.35572053
TAGACAG6200.08.2749925
GACCGTG1150.0011121648.2601627
TGCGTTG27550.08.13723512
GTCCTAT3400.08.1069711
GTCTTAG4600.08.0583531
GTGTAAG6350.07.9330771
TATTCCG1200.00169018337.91743045
GTTCTAG5050.07.90491961
GTCTTAT4850.07.83894821
Produced by FastQC (version 0.11.5)